The NovaSeq 6000 is the Genomic Center’s most recently acquired, most powerful next-generation sequencer, offering affordable ultra-deep sequencing, and the ability to approach problems from a whole-genome sequencing perspective. In order to assist UMN researchers in exploring the instrument’s capabilities and preparing grant applications that take advantage of its power, the Genomics Center and Illumina have partnered on a university-wide grant opportunity to support deep next-generation sequencing projects.
This competition is a two-step process that starts this spring with subsidized pilot sequencing, and winds up in the fall with two researchers receiving individual S2 flow cells valued at up to $21,000 each (or equivalent value in SP flow cells) . In the first phase, all qualifying researchers will be offered a promotional rate for one lane of sequencing on a NovaSeq S Prime (SP) flow cell, generating 400 M reads of preliminary data for a phase 2 S2 flow cell Sequencing Grant submission this fall. PIs will submit short, R01-style proposals including their phase 1 preliminary data, and two research projects will each be awarded an S2 flow cell (3.2 B reads).
We appreciat9e Illumina’s generous contribution of the sequencing reagents and S2 flow cells, and reduced costs for the SP flow cells. The Genomics Center will be providing sequencing labor free of charge for both grants.
Wednesday, March 13th: Begin accepting applications for the SP promotional rate.Friday, April 19th: GenoFling | Attend Illumina’s break-out session on applying for the S2 Sequencing Award (attendance not required to apply for the sequencing grant). Friday, April 26th (5:00 PM): Application deadline for the SP promotional rate. Friday, May 31 (5:00 PM): Samples or client-prepared libraries due for the initial pilot data phase. Monday, December 2nd (5:00 PM): Proposals due for the S2 Sequencing Grant. Monday, January 13th: Winners of the S2 flow cells will be notified via email.
Please contact us for questions or further clarification on any rules.
Qualifying projects can suggest any NGS application as the basis for their research, except for bulk RNA-Seq projects (single-cell RNA-Seq and using a combination of applications are acceptable). Libraries may be prepared by the researcher, or at the Genomics Center, but either option must have samples submitted by May 31st. Researchers must produce pilot data on the NovaSeq using the spring promotional SP rate in order to submit a proposal for the S2 Sequencing Grant in the fall.
The SP promotional rate and the S2 Sequencing Grant are open to UMN PIs. Applicants must hold primary research positions and be planning to submit a grant application to support a broader research aim for which they need pilot data. Applicants may not hold current awards directly related to the proposed research.
Only one application per PI will be accepted, and only applicants who use the spring SP promotional rate to generate pilot data are eligible to apply for the S2 Sequencing Grant this fall.
These two opportunities cover sequencing reagents and labor costs only. Researchers must be able to support the remaining costs of the SP lane, and library prep costs for both grants, plus and any other reagents and equipment needed to complete the project. Please contact firstname.lastname@example.org for a quote on library prep costs.
Researchers use the on-line application to apply for the SP promotional rate. General information about your S2 project will be needed, including sample depth, read length, application, and plans for informatics.
Application deadline: Friday, April 26th
Confirmation: Shortly after application submission
Deadline for sample submission: Friday, May 31st
The proposal for the S2 Sequencing Grant will be written using standard NIH-style format and is limited to six pages that must include Specific Aims (≤ 1 page), Background and Significance (≤ 1 pages), Preliminary Data (≤ 2 pages), and Research Design and Methods (≤ 2 page). The bibiolographjy will not be included in the page limit, and the Preliminary Data section must present the results form phase 1 SP pilot sequencing work.
Illumina will be hosting a break-out session at GenoFling on Friday, April 19th to provide further details on the proposal and answer any questions. Applicants will also be emailed the required sections and format for the proposal in early May. Note: The sooner samples are submitted, the sooner data can be released to your account and analyzed for the S2 proposal.
Proposal deadline: Monday, December, 2nd
For further clarification on any of these rules or questions, please contact us.