Oxford Nanopore: ultra-rapid nanopore-based single-molecule sequencing
Phase Genomics: Hi-C library methods for genome scaffolding, structural variant detection, and metagenomic genome assembly
Nabsys: solid-state nanodetectors for genome scaffolding and structural variant detection
We are honored that the scientific founders of two of these companies are coming in person to explain how their innovations can help advance science at the UMN.
We are also introducing a new UMGC program: UMGC-DIY. With this program, we are establishing a "tool-lending library" model for portable instruments such as the new Illumina iSeq sequencer, whereby UMN investigators will be able to lease a sequencer and other genomics instruments for use in their own lab. We will also be announcing expanded in-facility access to 384-well real-time PCR instruments, digital PCR instruments, and other tools. We will be awarding free periods of DIY instrument loan via lottery during GenoFling.
As usual, our other commercial partners (list to be announced later) will be on-hand with updates to their product lines.
The "UMGC-DIY" Program. Kenny Beckman, Ph.D., Director, UMGC
Real time DNA sequencing using Oxford Nanopore ‘Nanopore Sensing’ platform. Konstantinos (Gus) Potamousis, Field Scientist. Oxford Nanopore.
Oxford Nanopore Technologies’ platforms for the direct, electronic analysis of single molecules are changing how we sequence. Our instruments are adaptable for the detection and analysis of a range of analytes including DNA, RNA, proteins, and small molecules. Oxford Nanopore is also integrating data analysis into the standard workflow to provide real-time interpretation of data. Uses for our nanopore sensing platform include genome assembly, field-based applications, real-time pathogen detection and surveillance, metagenomic analysis, anti-microbial resistance detection, education, and many more.
From Contigs to Chromosomes: how Hi-C is transforming genome and metagenome assembly. Ivan Liachko, Ph.D., Founder & CEO, Phase Genomics, Inc.Chromosome conformation capture methods like Hi-C measure the 3D organization of DNA using a combination of in vivo crosslinking, proximity-ligation, and paired-end sequencing. The resultant information can be used to rapidly generate end-to-end chromosome scaffolds for large genomes and to detect large-scale structural variation. Since Hi-C junctions form within intact cells, any sequences interacting by Hi-C must have originated from the same species/strain in a mixed population, enabling metagenomic deconvolution. Proximity-ligation methods enable whole genome scaffolding, microbial discovery, and structural variation detection using only conventional lab equipment and short-read sequencing.
Coffee Break + Vendor Fair Part I
Nabsys high-definition mapping using high-speed semiconductor-based nanodetectors. John S. Oliver, Ph.D., Chief Technology Officer, Nabsys.Nabsys HD mapping uses semiconductor-based nanodetectors to analyze long 100kb+ DNA molecules traveling at high velocity (1 megabase per second). The approach allows for the analysis of structural variation to be cost-effective enough to be done as routinely as the analysis of single-nucleotide variation. Applications include genome-wide analysis of structural variation, large and small genome assembly, and microbiome analysis.
GenoPitch CompetitionPromotion: two $2,500 genomics pilot projects Awarded: via audience vote during GenoPitch
Catered Lunch and Vendor Fair Part II