The UMGC is a multi-facility resource encompassing services for sequencing, fragment analysis, genotyping, gene expression analysis, epigenomics, structural genomics, RNAi knockdown, oligonucleotide synthesis, single-cell genomics, microbiome studies, and CLIA-based Next-generation Sequencing.
A staff of 38 scientists is housed in a total of 6 laboratories spanning the Twin Cities campuses. Together, these core facilities encompass over 6,000 square feet. The UMGC has a budget of > $8M per year and hundreds of internal and external clients.
ABI 3730xl DNA Analyzer + Beckman NX 96-well liquid handling robot for automated reaction set up.
Illumina NovaSeq. Two Illumina NextSeqs. One Illumina HiSeq 2500 High-output with one cBot cluster generation robot. Five Illumina MiSeq sequencers. One iSeq 100.
PacBio Sequel System, 10X Genomics, Oxford Nanopore GridION X5.
Covaris S220 Acoustic DNA Shearing Device. Caliper LabChip XT and Sage Biosciences PippinPrep and ELF automated gel-sizing devices. Fluidigm Access Array system for amplification-based NGS library creation. 10X Genomics Chromium platform for linked-read (phased haplotype) NGS.
One Fluidigm C1 microfluidic cell capture and processing system. 10X Genomics Chromium platform for high-throughput single-cell transcriptomics.
Two ABI 7900HT 384-well real-time PCR instruments (1 with autoloader) for uniplex (Taqman) genotyping; Agena MassARRAY System platform; Illumina Platform including iScan scanner and autoloader and Tecan Infinium automation accessory equipped for Infinium genotyping chemistries.
Illumina Platform including Illumina iScan scanner and autoloader and Tecan Infinium automation accessory equipped for Infinium DNA methylation chemistries. DNA methylation analysis using Next-generation Sequencing on Illumina HiSeq and MiSeq sequencers in combination with bisulfite sequencing and/or locus-specific sequence capture. PacBio Sequel DNA methylation sequencing.
Two ABI 7900HT 384-well real-time PCR instruments (1 with autoloader) for real- time Q-PCR analysis, and full complement of Roche Universal Probe Library (UPL) probes; Illumina Platform including Illumina iScan scanner and autoloader for Illumina DirectHyb and DASL expression chemistries; NanoString nCounter Gen2 platform. Fluidigm BioMark HTP real-time Q-PCR/ digital PCR platforms. Bio-Rad QX200 Droplet Digital PCR system.
Over 40 PCR thermocyclers blocks (96- + 384-well); QIAGEN, Beckman, Eppendorf, and Hydra liquid handling robots, Agilent 2100 Bioanalyzers (3), 8-channel Nanodrop quantitation instruments (2), Caliper GX and Agilent TapeStation 2000 high-throughput capillary electrophoresis system.
The UMGC has built and operates its own electronic Laboratory Information Management System (LIMS). For most services, projects are ordered online, and data are posted electronically. MSI’s Research Informatics Solutions (RIS) and the University of Minnesota Informatics Institute provide bioinformatic assistance.
In addition, the UMGC maintains a library of ≈ 300,000 shRNA knockdown constructs for use by UMN researchers for manipulation of gene expression levels in human and mouse cell lines. We provide clones, lentiviral preps, and library pools of these clones.