Exposure of denatured DNA to sodium bisulfite results in the conversion of the base cytosine (C) – but not methylated cytosine (C*) – to uracil (U). Subsequent amplification of treated DNA by PCR (or any other DNA polymerase based method) converts U to T. Consequently, bisulfite conversion results in the change of C residues in DNA to T, leaving *C unchanged. Once such conversion has been accomplished, C/C* ratios can be measured as T/C ratios, using any tools designed for quantification of DNA variants. Bisulfite conversion is therefore a critical first step in many technologies for the study of DNA methylation. We use Zymo Research Corporation EZ DNA Methylation Kit for bisulfite conversion, either in combination with other services (See Infinium MethylationEPIC), or as a standalone service.
Contact Shalane Porter: firstname.lastname@example.org
|Bisulfite Conversion of DNA.|
|A submission of 12 samples is the minimum scale accepted.||12-14
|Bisulfite Conversion.||From tissues. Individual Spin column.||1-any||sample||$17.25||$19.86|
Complete the DNA Plate Submission Form.
For University researchers, submit the completed form through the online ordering system by creating an order for “Genotyping, Epigenomics and CNV Analysis” by clicking on the “SNP and Sample Submission Form”. For researchers outside the University, submit the completed form to email@example.com.
Researchers should Submit a minimum of 500 ng of DNA per sample at 25 ng/ul in a 96-well plate in a COLUMN-WISE fashion RANDOMIZING samples and keeping two unique blank wells. Please review our MethylationEPIC Sample Submission Form Guidelines.
1-202 Nils Hasselmo Hall (Minneapolis campus)
1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
20 Snyder Hall (St. Paul campus)
Please send the tracking information to firstname.lastname@example.org.
University of Minnesota Genomics Center Genotyping 312 Church St SE, 1-202 Nils Hasselmo Hall Minneapolis, MN 55455 612-624-3177