Olink biomarker panels enable high-throughput, multiplex immunoassays that measure 92 proteins across 96 samples simultaneously using one microliter of serum, plasma, or almost any other type of biological sample.
A pair of oligonucleotide-labeled antibodies are allowed to pair-wise bind to a target protein present in the sample in a homogeneous assay with no need for washing. When the two Proseek probes are in close proximity, a new PCR target sequence is formed by a proximity-dependent DNA polymerization event. The resulting sequence is subsequently detected and quantified using standard real-time PCR.
Each of the 96 oligonucleotide antibody-pairs contains unique DNA sequences allowing hybridization only to each other. Cross-reactive events will not be detected with Olink’s panels since only matched DNA reporter pairs are amplified with real-time PCR. This allows for scalable multiplexing without loss of specificity and sensitivity.
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