The innovative Chromium instrument from 10X Genomics is an emulsion-tagging library creation platform that can be used for both DNA-based and single-cell RNA-based applications. The Chromium technology uses >1,000,000 unique barcodes for highly parallel sample partitioning and molecular barcoding to generate Illumina-ready libraries to be sequenced on our HiSeq2500. The resulting short-read NGS output files are fed into 10X Genomics’ analysis pipelines.
For DNA Chromium creates “linked reads” (barcoded sets of reads) from single, ultra-long molecules; the barcodes are used to link sequencing reads to the originating molecule. These linked reads can be used for whole-genome phasing, structural variant detection, and de novo genome assembly.
In its single-cell genomics format, the 10X Chromium uniquely tags transcripts from single cells to enable affordable single-cell gene expression analysis of between 200-10,000 cells per run.