The C1 system is an automated solution for studying heterogeneity within cell populations. Fluidigm’s integrated fluidic circuit (IFC) enables isolation, imaging, and processing of individual cells. The individual cells are further characterized utilizing real-time qPCR (Fluidigm BioMark HD) or next-generation sequencing (RNA-Seq, 3’ mRNA gene counting, DNA-Seq, and ATAC-Seq).
To schedule a capture on the C1, please visit: https://docs.google.com/spreadsheets/d/1hYPbja5wB3zOqpfj6Ug__GrZ9qhZeQKkNVGioTAWIaE/pubhtml
The Fluidigm BioMark HD is a microfluidic, high-throughput instrument for real-time Q-RT-PCR of up to 9,216 reactions in a single run. The heart of the platform is a microfluidic chip called an “Integrated Fluidic Circuit (IFC)” into which are deposited amplification-ready templates (e.g., cDNA) and gene-specific assays (e.g., Taqman).
The IFC performs automatic mixing of templates and assays in nanoliter amplification wells, followed by real-time PCR and data capture on the BioMark HD.
High-throughput IFCs can be run at the UMGC in three formats (# templates vs # assays): 1) 96.96 (9,216 reactions), 2) 48.48 (2,304 reactions) and 3) 192.24 (4,608 reactions). A fourth IFC also exists – the “FLEXsix IFC” – containing six independent 12.12 arrays that can be run independently for small-scale experiments or pilot runs.
The IFC also features targeted next-generation library preparation of DNA through the Access Array System. The Access Array creates amplicon libraries using sample-specific barcode sequences attached to each PCR product. Multiplex primers can build libraries for a few user defined genes up to panels of up to 4,800 amplicons, and barcode sequences let you run up to 384 samples in one sequencing run. Please contact UMGC staff (email@example.com) if you are interested in more information or to request a quote for this service.To read more about the Biomark HD, please visit: https://www.fluidigm.com/products/biomark-hd-system