The UMGC currently operates three HiSeq 2500s in our CCRB location. HiSeq 2500 instruments can be run in two different modes: “high output” or “rapid run”. High output mode utilizes 8-lane flow cells in combination with “version 4 chemistry”, whereas rapid run mode uses 2-lane flow cells with “rapid” SBS chemistry. Only our most recently acquired HiSeq 2500 (“Franklin”) is configured to run in high output mode using v4 chemistry. Two of our HiSeq 2500s (“Watson” and “Crick”) use Illumina’s “rapid” SBS chemistry on two-lane flow cells.
For more information on the HiSeq 2500, please visit: http://www.illumina.com/systems/hiseq_2500_1500.html
Currently, we possess five MiSeqs that operate in two of our laboratory locations. The Illumina MiSeq is a “desktop” sequencer specialized for quick, lower-output runs on single-lane flow cells. With only a single lane to scan, the MiSeq has the quickest run per cycle of any Illumina instrument, and consequently, achieves the longest read length (paired-end 300 cycles). The MiSeq is therefore ideal for targeted sequencing of long amplicons, including microbial 16S sequencing.
For more information on the MiSeq, please visit: http://www.illumina.com/systems/miseq.html
The UMGC currently operates two cBots in two of our laboratory locations. The cBot amplifies libraries to produce clonal clusters that are sequencer ready.
To read more about the cBot, please visit: http://www.illumina.com/documents/products/datasheets/datasheet_cbot.pdf
The iScan system can be configured to different scales depending on project requirements. In addition to the iScan, the UMGC has a Tecan liquid handling robot, Autoloader 2.x, and hybridization oven which allows continual scanning throughout the day.
For more information on the iScan system, please visit: http://www.illumina.com/systems/iscan.html