The UMGC is pleased to now offer long-read sequencing on the PacBio Sequel platform. Released in mid-2016, the Sequel is PacBio’s most recent instrument, and uses single-molecule real-time (SMRT) technology to generate reads with modal lengths of 8-10 kb. With recently introduced “V2” chemistry and “V4” software, mean reads lengths are reported to increase to 10-18 kb, with half of data in reads > 20 kb and read lengths up to 80 kb possible. One SMRT cell “run” on the Sequel generates between 5-8 Gb of primary data. The Sequel’s long reads are particularly suited to genome assembly, microbial genomics, detection of structural variants, and transcriptional isoform sequencing (Iso-Seq).
For more information on the Sequel System, please visit: http://www.pacb.com/products-and-services/pacbio-systems/sequel/