HiSeq 2500 instruments can be run in two different modes: “high output” or “rapid run”. high output mode utilizes 8-lane flow cells in combination with “version 4 chemistry”, whereas rapid run mode uses 2-lane flow cells with “Rapid” SBS chemistry. Only our most recently acquired HiSeq 2500 (“Franklin”) is capable of running in high output mode.
For single-read runs, the UMGC guarantees ≥ 220 million reads in the forward direction. For paired end runs we guarantee ≥ 220 million reads in both forward and reverse directions (resulting in ≥ 440 million single reads in all). Output metrics are guaranteed for "standard" RNA or DNA libraries. Custom libraries, amplicon libraries or low diversity libraries are not subject to this read guarantee.
High-output mode provides almost twice as many reads per lane than rapid-run mode, at a lower cost per lane, resulting in a cost per read about half that of rapid run mode.
Thanks to the use of 8-lane flow cells, it can take considerably longer for a high output mode run to be “filled” with enough samples for a run to proceed. As a result, project turnaround in high output mode can be longer than in rapid-run mode.
Contact Aaron Becker: firstname.lastname@example.org.
|HiSeq 2500 High Output Mode – Single-read|
|50-bp Single-read (1x50 SR).1||1-any||lane||$1,025.40||$1,455.52|
|100 bp Single-read (1 x 100 SR).2||1-any||lane||$1,346.00||$1,910.61|
|HiSeq 2500 High Output Mode – Paired-end|
|50-bp Paired-end (2x50 PE).3||1-any||lane||$1,642.95||$2,332.12|
|125-bp Paired-end (2x125 PE).4||1-any||lane||$2,368.76||$3,362.39|
Researchers can download the appropriate submission form for submitting samples or submitting client-made libraries. Once the form has been filled, please complete the submission by emailing email@example.com.
1-202 Nils Hasselmo Hall (Minneapolis campus)
1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
20 Snyder Hall (St. Paul campus)
Please send the tracking information to firstname.lastname@example.org.
University of Minnesota Genomics Center
1475 Gortner Ave.
28 Snyder Hall
St. Paul, MN 55108
There are three options for transferring data from the UMGC to clients: delivery to the Minnesota Supercomputing Institute’s (MSI) high-performance file system, download from a secure website, or shipment on an external hard drive. Please indicate your data delivery preference when placing a order for sequencing.
Internal clients and external clients that opt-in to MSI storage have their data released to the client’s MSI group directory on the MSI high-performance filesystem (/home/GROUP/data_release/umgc), from where it can be analyzed using MSI’s high-performance computers or Galaxy system. The data is stored for five years in uncompressed format with read-only permissions to avoid accidental modification, and secure off-site tape backups of the data are maintained for disaster recovery. MSI will charge the client a storage fee for this service.
Internal clients that opt-out of MSI storage and external clients can download their data from a secure website using either a web browser or a command-line download tool, complete instructions are provided in an email from the UMGC. The client’s data is available for download for 30 days, after which the data will be removed from the data download website and the client takes responsibility for storing the data.
External clients may have data shipped on a hard drive purchased by the UMGC and invoiced to the client.
MSI guarantees storage of client data for five years, after which the data can be transferred to tape for long-term storage, contact MSI (email@example.com) for more information.
Data is typically recoverable for up to 5 years. If the external researcher should ever need the FASTQ files re-posted to the data download website or shipped on a hard drive within 5 years from data release, email firstname.lastname@example.org to initiate data recovery. The client will be charged for the cost of recovering the data from tape archive. The UMGC does not provide any guarantee that data can be successfully recovered from tape archive.
Yes, please consult with UMGC staff before submitting client-made libraries by emailing email@example.com. We recommend that pooling be done at UMGC as PicoGreen concentrations are used for balancing libraries. However, clients may choose to pool themselves.
Client-made libraries will have the following services performed on them before proceeding with sequencing.
University of Minnesota Genomics Center 1475 Gortner Ave. 28 Snyder Hall St. Paul, MN 55108 612-625-7736