Two of our HiSeq 2500s (“Watson” and “Crick”) use Illumina’s “Rapid” SBS chemistry on two-lane flow cells.
For single-read runs, the UMGC guarantees ≥ 120 million reads in the forward direction. For paired end runs, we guarantee ≥ 120 million reads in both forward and reverse directions (resulting in ≥ 240 million single-reads in all).
With only two flow cell lanes to scan, rapid runs are fast, enabling quicker turnaround and the longest read lengths available (2x250 bp). Moreover, with only two lanes to occupy, rapid run flow cells are easier to “fill”, with less delay incurred waiting for samples to be submitted for sequencing. As a result, project turnaround in rapid run mode can be shorter, especially for sequencing read lengths that are less commonly requested.
Due to the smaller surface area of rapid run lanes, output is lower – and cost per read higher – than high output mode.
Contact Aaron Becker: firstname.lastname@example.org.
|HiSeq 2500 Rapid Run Mode – Single-read|
|50-bp Single-read (1x50 SR).1||1-any||lane||$1,443.36||$2,048.67|
|100 bp Single-read (1 x 100 SR).2||1-any||lane||$1,739.14||$2,468.67|
|HiSeq 2500 Rapid Run Mode Mode – Paired-end|
|50-bp Paired-end (2x50 PE).3||1-any||lane||$2,013.01||$2,857.42|
|100-bp Paired-end (2x100 PE).4||1-any||lane||$2,493.93||$3,540.07|
|150-bp Paired-end (2x150 PE).5||1-any||lane||$3,085.46||$4,379.74|
|250-bp Paired-end (2x250 PE).6||1-any||lane||$3,804.16||$5,399.92|
Researchers can download the appropriate submission form for submitting samples or submitting client-made libraries. Once the form has been completed, please submit the submission by emailing email@example.com.
1-202 Nils Hasselmo Hall (Minneapolis campus)
1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
20 Snyder Hall (St. Paul campus)
Please send the tracking information to firstname.lastname@example.org.
University of Minnesota Genomics Center
1475 Gortner Ave.
28 Snyder Hall
St. Paul, MN 55108
There are three options for transferring data from the UMGC to clients: delivery to the Minnesota Supercomputing Institute’s (MSI) high-performance file system, download from a secure website, or shipment on an external hard drive. Please indicate your data delivery preference when placing a order for sequencing.
Internal clients and external clients that opt-in to MSI storage have their data released to the client’s MSI group directory on the MSI high-performance filesystem (/home/GROUP/data_release/umgc), from where it can be analyzed using MSI’s high-performance computers or Galaxy system. The data is stored for five years in uncompressed format with read-only permissions to avoid accidental modification, and secure off-site tape backups of the data are maintained for disaster recovery. MSI will charge the client a storage fee for this service.
Internal clients that opt-out of MSI storage and external clients can download their data from a secure website using either a web browser or a command-line download tool, complete instructions are provided in an email from the UMGC. The client’s data is available for download for 30 days, after which the data will be removed from the data download website and the client takes responsibility for storing the data.
External clients may have data shipped on a hard drive purchased by the UMGC and invoiced to the client.
MSI guarantees storage of client data for five years, after which the data can be transferred to tape for long-term storage, contact MSI (email@example.com) for more information.
Data is typically recoverable for up to 5 years. If the external researcher should ever need the FASTQ files re-posted to the data download website or shipped on a hard drive within 5 years from data release, email firstname.lastname@example.org to initiate data recovery. The client will be charged for the cost of recovering the data from tape archive. The UMGC does not provide any guarantee that data can be successfully recovered from tape archive.
University of Minnesota Genomics Center 1475 Gortner Ave. 28 Snyder Hall St. Paul, MN 55108 612-625-7736