Nano runs ≥ 1 million reads. (2x150 bp, 2x250 bp)
Micro runs ≥ 4 millions reads. (2x150 bp only)
Version 2 chemistry ≥ 8 millions reads. (1x50 bp, 2x150 bp, 2x250 bp)
Version 3 chemistry ≥ 16 million reads. (2x75 bp, 2x300 bp)
Stowaway Sequencing ≈ 1-2 million reads. (2x300 bp only)
With only a single lane to scan, the MiSeq has the quickest run per cycle of any Illumina instrument, and consequently, achieves the longest read length (paired-end 300 cycles). The MiSeq is therefore ideal for targeted sequencing of long amplicons, including microbial profiling. Turnaround time on the MiSeq is also theoretically faster than on other Illumina instruments (although in practice, queues may develop on the instrument). Our expedited 2x300-bp run is designed for projects requiring guaranteed 1-week turnaround. We also offer a shared MiSeq run called Stowaway-Seq.This is a service designed to provide shallow (≤ 2M reads), low-cost ($299.09) sequencing for small-scale pilot experiments.
Cost per read is significantly higher on the MiSeq platform than on HiSeq instruments.
Contact Aaron Becker: firstname.lastname@example.org.
|MiSeq Nano – Paired-end|
|150-bp Paired-end (2x150 PE).||1-any||lane||$876.74||$1,064.69|
|250-bp Paired-end (2x250 PE).||1-any||lane||$1,007.21||$1,233.14|
|150-bp Paired-end (2x150 PE).||1-any||lane||$1,197.56||$1,454.29|
|MiSeq. Version 2 – Single-read.|
|50-bp Paired-end (1x50 SR).||1-any||lane||$1,136.50||$1,380.14|
|MiSeq. Version 2 – Paired-end|
|150-bp Paired-end (2x150 PE).||1-any||lane||$1,389.10||$1,686.89|
|250-bp Paired-end (2x250 PE).||1-any||lane||$1,518.39||$1,843.90|
|MiSeq. Version 3 – Paired-end|
|75-bp Paired-end (2x75 PE).||1-any||lane||$1,228.68||$1,492.09|
|300-bp Paired-end (2x300 PE).||1-any||lane||$1,968.50||$2,390.52|
|300-bp Paired-end (2x300 PE). Expedited||1-any||lane||$3,210.19||$3,698.95|
|1/8 shared lane of MiSeq 300-bp Paired-end (2x300 PE).||1-any||lane||$299.09||$391.81|
Researchers can download the appropriate submission form for submitting samples or submitting client-made libraries. Once the form has been completed, please submit the submission by emailing email@example.com.
1-202 Nils Hasselmo Hall (Minneapolis campus)
1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
20 Snyder Hall (St. Paul campus)
Please send the tracking information to firstname.lastname@example.org.
University of Minnesota Genomics Center
1475 Gortner Ave.
28 Snyder Hall
St. Paul, MN 55108
There are three options for transferring data from the UMGC to clients: delivery to the Minnesota Supercomputing Institute’s (MSI) high-performance file system, download from a secure website, or shipment on an external hard drive. Please indicate your data delivery preference when placing a order for sequencing.
Internal clients and external clients that opt-in to MSI storage have their data released to the client’s MSI group directory on the MSI high-performance filesystem (/home/GROUP/data_release/umgc), from where it can be analyzed using MSI’s high-performance computers or Galaxy system. The data is stored for five years in uncompressed format with read-only permissions to avoid accidental modification, and secure off-site tape backups of the data are maintained for disaster recovery. MSI will charge the client a storage fee for this service.
Internal clients that opt-out of MSI storage and external clients can download their data from a secure website using either a web browser or a command-line download tool, complete instructions are provided in an email from the UMGC. The client’s data is available for download for 30 days, after which the data will be removed from the data download website and the client takes responsibility for storing the data.
External clients may have data shipped on a hard drive purchased by the UMGC and invoiced to the client.
MSI guarantees storage of client data for five years, after which the data can be transferred to tape for long-term storage, contact MSI (email@example.com) for more information.
Data is typically recoverable for up to 5 years. If the external researcher should ever need the FASTQ files re-posted to the data download website or shipped on a hard drive within 5 years from data release, email firstname.lastname@example.org to initiate data recovery. The client will be charged for the cost of recovering the data from tape archive. The UMGC does not provide any guarantee that data can be successfully recovered from tape archive.
Yes, please consult with UMGC staff before submitting client-made libraries by emailing email@example.com.
We recommend that pooling be done at UMGC as PicoGreen concentrations are used for balancing libraries. However, clients may choose to pool themselves.
Client-made libraries will have the following services performed on them before proceeding with sequencing.
University of Minnesota Genomics Center 1475 Gortner Ave. 28 Snyder Hall St. Paul, MN 55108 612-625-7736