Marker gene profiling using the 16S rRNA gene, 18S rRNA gene, or Internal Transcribed Spacer (ITS) region is commonly used to study microbial diversity. The UMGC provides two workflows: A UMGC-developed dual-indexing approach, and the Earth Microbiome Project protocol.
We offer library prep for multiple marker gene variable regions (bacterial 16S rRNA V1-V3, V3-V4, V3-V5, V4, V4-V6, V5-V6, eukaryotic ITS1/ITS2, and eukaryotic 18S rRNA V9), using UMGC-developed methods, which in a recent report in Nature Biotechnology were shown to provide more quantitatively accurate and qualitatively complete measurement of mock community reference standards than other commonly used protocols. This protocol uses a two step process in which the marker gene of interest is first amplified using a set of adapter-tailed primers, followed by addition of flow cell adapters and sample-specific indices by overlap extension.
The Earth Microbiome Project (EMP) protocol is available for the bacterial 16S rRNA V4 region, and the eukaryotic ITS1 region. This protocol uses a single round of PCR amplification during which flow cell adapters and sample-specific indices are added to the PCR primers to enable multiplexing of samples.
Standard turnaround (submission to NGS data release) is typically within 4-6 weeks of sample submission. In cases where a more rapid turnaround is required, our expedited service may be available, with data returned in 7 days or less from the time of submission.
Contact Allison MacLean: firstname.lastname@example.org
|16S/18S/ITS. UMGC Dual-indexing.|
|Price per sample. Variable regions available include: bacterial V1-V3, V3-V4, V3-V5, V4, V4-V6, V5-V6, and eukaryotic 18S (V9), ITS1, and ITS2. Standard turnaround is within 4-6 weeks of sample submission.||1-any||sample||$10.95||$12.65|
|16S/18S/ITS. UMGC Dual-indexing. Expedited.|
|Price per 96-well plate. Turnaround from submission to data release ≤ 7 days. This rate must be paired with Expedited MiSeq 300-bp Paired-end 2x300 PE) run.||1||up to 94
|Earth Microbiome Project Protocol (V4 and ITS1).|
|Price per sample. V4 and ITS1 regions only.||1-any||sample||$8.49||$9.84|
Please contact email@example.com for project specifications.
Once project details are finalized, complete appropriate submission form for submitting samples or submitting client-made libraries and email to firstname.lastname@example.org.
1-202 Nils Hasselmo Hall (Minneapolis campus)
1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
20 Snyder Hall (St. Paul campus)
Please send the tracking information to email@example.com.
University of Minnesota Genomics Center
312 Church Street NE
1-202 Nils Hasslemo Hall
Minneapolis, MN 55455
Standard turnaround (submission to NGS data release) is typically within 4-6 weeks of sample submission. In cases where a more rapid turnaround is required, our expedited service may be available, with data returned in 7 days or less from the time of submission. Please contact firstname.lastname@example.org for specific project turnaround time.