Next-generation sequencing is a powerful means to profile DNA methylation at the single-nucleotide resolution when it is combined with bisulfite conversion of DNA either before or after library creation.
Exposure of DNA to sodium bisulfite results in the conversion of cytosine (C) – but not methylated cytosine (C*) – to uracil (U). Subsequent amplification of DNA during PCR converts U to T. Consequently, bisulfite conversion results in the change of C residues in DNA to T, leaving *C unchanged. Once such conversion has been accomplished, the original methylation state – C or C* – can be read out as the presence of a C vs T at a given CpG site.
Contact Aaron Becker: firstname.lastname@example.org.
|Reduced Representation Bisulfite Sequencing (RRBS).¹|
|A “traditional” DNA methylation library creation||1-12||sample||$235.66||$272.18|
|method utilizing bisulfite conversion after||13-48||sample||$165.06||$194.14|
|standard DNA shearing-based library creation.||49-any||sample||$137.86||$164.42|
|The method uses kits and protocols from New England Biolabs.|
|1. There are a large variety of other methods for the measurement of DNA methylation in DNA, including whole-genome methods, methods based on sequence capture of CpG islands, and targeted methods. Please inquire for more details. (email@example.com)|
Please contact firstname.lastname@example.org for project specifications.
Once project details are finalized, complete appropriate submission form for submitting samples or submitting client-made libraries and email to email@example.com.
Samples should be frozen and shipped on dry ice in a 96-well plate. We recommend using plate tape to seal the wells. Place the plate inside of a plastic bag prior to placing on dry ice. Please give advance notice of submission date and time so staff can be prepared to receive samples. If shipping samples from outside the University of Minnesota, ship via express shipping carrier on dry ice to the address below:
1-202 Nils Hasselmo Hall (Minneapolis campus)
1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
20 Snyder Hall (St. Paul campus)
Please send the tracking information to firstname.lastname@example.org.
University of Minnesota Genomics Center
1475 Gortner Avenue
28 Snyder Hall
St. Paul, MN 55108
Generally, turnaround is 6-8 weeks for standard NGS projects. However, please contact email@example.com for specific project turnaround time.