DNA Extraction
The UMGC offers DNA extraction using a wide range of commercially available kits, bead beating methods and homebrew protocols developed at the UMGC for high-throughput extraction in 96-well plate format.
We also offer RNA Extraction.
We routinely extract DNA from the following tissue sources. If you have tissue not listed below, please contact us.
- Whole Blood – The UMGC processes bulk whole blood samples for gDNA using a “salting out” method that has been in place at the UMN’s hospital clinic for two decades, and delivers very high yields.
- Saliva – Oragene Collection kits
- Buccal swabs
- FTA filter paper
- Plant and Animal Tissue
- Environmental and Fecal
- Cell Pellets Bacteria
The assessment of DNA purity and concentration by UV spectroscopy (NanoDrop) and fluorimetry (PicoGreen quantification) is included in the price of most services.
Questions?
Contact Dinesha Walek: walek001@umn.edu.
| Service | Scale | Unit | UMN | External | ||
|---|---|---|---|---|---|---|
| Whole blood. 1-3 Vacutainer Tubes. | ||||||
| Yields from 1-3 Vacutainer tubes are typically in excess of 250 ug. | 1-96 | sample | $24.05 | $27.78 | ||
| 1-36 | sample | $14.80 | $17.44 | |||
| QIAGEN DNeasy Blood & Tissue Kit. | ||||||
| Yields are typically in the range of 2-10 ug. | 1-36 | sample | $14.98 | $17.28 | ||
| 37-any | sample | $9.93 | $11.68 | |||
| Oragene Buccal Cell Kit. | ||||||
| Please note, the kits are purchased by the researcher through Oragene. | 1-95 | sample | $32.17 | $37.21 | ||
| 96-any | sample | $25.96 | $30.33 | |||
| “Quick-and-Easy” for Mouse Genotyping. | ||||||
| Tissue samples (mouse tail tip, toe or ear clippings) may be submitted in 96-well PCR plates completely covered in 100% ethanol. Please note, this method forgoes certain quantification and purification steps when unnecessary for downstream processing. | 1-any | sample | $1.04 | $1.20 | ||
| MoBio PowerSoil for Stool, Soil, and Other Environmental Samples. | ||||||
| Yields depend on sample type. | 1-95 | sample | $24.65 | $28.35 | ||
| 96-any | sample | $12.94 | $15.16 | |||
| High-throughput Plant Extraction. | ||||||
| Minimum submission is one plate of plant material. Note that a pilot extraction using this homebrew UMGC method will be required for any species or tissue type/format that the UMGC has not yet encountered. | 1-any | plate of 96 | $631.62 | $730.32 | ||
How to Order
Please contact extract@umn.edu for project specifications.
Once project details are finalized, complete the nucleic acid extraction sample submission form and email to extract@umn.edu.
Samples can be dropped-off at one of our laboratory locations.
- 1-202 Nils Hasselmo Hall (Minneapolis campus)
- 1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
- 20 Snyder Hall (St. Paul campus)
If shipping samples, the following address should be used.
Please send the tracking information to extract@umn.edu.
University of Minnesota Genomics Center
312 Church Street SE
Attn: Dinesha Walek/Jiangming Deng
1-202 NHH
Minneapolis, MN 55455
612-624-3177
Deliverable
Client receives a QC report with sample details.
What is the turnaround time?
Please contact walek001@umn.edu for specific project turnaround time.
What is the turnaround time?
It depends upon sample numbers. We handle both small (1-2 samples) and large (in the thousands). Please contact us for a timeline.
What is your recommendation on shipping?
- Dry Ice – Cells, Tissue, Blood, Fecal and Plant
- Room Temp – Buccal Dried Swabs, Oragene kits, and FTA cards
What is your shipping address?
Attn: Dinesha Walek/Jiangming Deng
312 Church Street SE
1-202 NHH
Minneapolis, MN 55455
How do determine quality of extracted DNA?
The assessment of DNA purity and concentration is by both UV spectroscopy (NanoDrop) and fluorimetry (PicoGreen quantification), usually included in most of DNA extraction services. Upon request, we can also provide DNA sizing either via Gel or Agilent Bio-analyzer.
What type extractions do you offer?
We use a wide range of commercially available kits, bead beating methods as well as Home-Brew methods, listed below are some common methods. If you have your own method please contact us.
- QIAGEN DNeasy: a standard, lower-cost silica-binding purification method that provides DNA of excellent quality for genomic applications.
- ORAGENE BUCCAL: Oragene buccal cell collection kits offer pain-free and noninvasive collection that is readily self-administered, suitable for shipment in standard mail, and stable at room temperature.
- QUICK-AND-EASY: low-cost extraction suitable for downstream mouse genotyping. The output is a semi-purified DNA prep that compatible with PCR-based assays. The method forgoes quantification and purification in order to achieve the lowest cost possible.
- QIAGEN DNeasy POWERSOIL (formerly MOBIO POWERSOIL): a “bead-beating” method that uses abrasive beads to break the cell walls of microbes in stool and other samples. This service is typically used in combination with 16S/18S sequencing.
- HIGH-THROUGHPUT PLANT: a homebrew method developed at the UMGC for high-throughput extraction of DNA from plant material submitted in 96-well plate format. Please inquire for details.
- QIAGEN MagAttract PowerMicrobiome DNA/RNA: a “bead-beating” method that uses abrasive beads to break the cell walls of microbes in stool and other samples. Extracts both RNA and DNA This service is typically used in combination with 16S/18S/Std NGS Seq sequencing.
Can you Store our samples?
UMGC has the capability of archiving your sample with 2D-Barcoded tubes/Plates and a retrieval system. This also enables us to re-rack/cherry pick samples for future downstream applications at the UMGC or ship them to an off-site location.