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Sagner Sequencing
Sanger Sequencing Classic Sanger Quality Check and Sequencing Clean and Load Only Custom
  1. Services
  2. Sanger Sequencing

Sanger Sequencing


The UMGC provides Sanger sequencing on an Applied Biosystems 3730xl DNA Analyzer. This 96-capillary instrument affords rapid, high-throughput service with read lengths of up to 800-1000 bp (with performance depending on the quality of the template and the sequence context). Our Sanger services are geared to the need for low-cost, rapid, routine sequencing of template-primer reactions assembled by clients (“Classic” service), although we also able to work with clients to optimize difficult-to-sequence regions.

All services use ABI BigDye Terminator version 3.1 chemistry.

Questions


Contact Patrick Warner: umgcseq@umn.edu


  Service Option   Repeat reaction
if failure
Include
hard copy?
Includes quantification  
 
  Classic Sequencing.
Tube Submission.
  Yes Yes No







  Classic.
Plate Submission.
  No No No







  Sanger Quality Check and Sequencing. Tube Submission.   Yes Yes Yes







  Sanger Quality Check and Sequencing. Plate Submission.   Yes No Yes







  Clean and Load Only   No No No







  Custom Option Sequencing.   Yes, except for
BAC & gDNA
Yes No







Nils Hasselmo Hall

312 Church Street SE
Minneapolis, MN 55455
612-624-3177
M-F 8:00 AM - 4:30 PM

CCRB

2231 6th Street SE
Minneapolis, MN 55455
612-624-6269
M-F 8:00 AM - 4:30 PM

Snyder Hall

1475 Gortner Avenue
St. Paul, MN 55108
612-625-7736
M- F 8:00 AM - 4:30 PM

Dr. Kenneth Beckman, Dir.

2231 6th Street SE
Minneapolis, MN 55455
Phone: 612-626-2985
kbeckman@umn.edu

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