University of Minnesota Genomics Center

Sanger Sequencing

The UMGC provides Sanger sequencing on an Applied Biosystems 3730xl DNA Analyzer. This 96-capillary instrument affords rapid, high-throughput service with read lengths of up to 800-1000 bp (with performance depending on the quality of the template and the sequence context). Our Sanger services are geared to the need for low-cost, rapid, routine sequencing of template-primer reactions assembled by clients (“Classic” service), although we also able to work with clients to optimize difficult-to-sequence regions.

All services use ABI BigDye Terminator version 3.1 chemistry.

Questions

Contact Patrick Warner: umgcseq@umn.edu

Service Option	Repeat reaction if failure	Include hard copy?	Includes quantification

Classic Sequencing. Tube Submission.	Yes	Yes	No

Classic. Plate Submission.	No	No	No

Sanger Quality Check and Sequencing. Tube Submission.	Yes	Yes	Yes

Sanger Quality Check and Sequencing. Plate Submission.	Yes	No	Yes

Clean and Load Only	No	No	No

Custom Option Sequencing.	Yes, except for BAC & gDNA	Yes	No