When combined with RNA-Seq, the Fluidigm C1 provides the ability to analyze the transcriptomes of dozens to hundreds of single-cells.
Microscope access at the University Imaging Center is required to inspect capture efficiency. Users should plan to be on-hand immediately following capture in order to view cells and make go/no-go decision on proceeding with downstream processing.
A 96-cell Fluidigm C1 + RNA-Seq project includes three costs: 1) C1 capture/cDNA creation (on-chip), 2) RNA-Seq library creation (off-chip), and 3) sequencing per se. On the pricing pane, prices for capture/cDNA and library creation are listed (for sequencing, see the Next-generation Sequencing section).
An 800-cell Fluidigm C1 RNA-Seq project includes two costs: 1) C1 capture/cDNA/RNA-Seq library creation (on chip), and 2) sequencing per se (for sequencing, see the Next-generation Sequencing section). On the Pricing pane, prices for capture/cDNA/library creation is listed (for sequencing, Next-generation Sequencing section).
Contact Jerry Daniel: firstname.lastname@example.org
|Single-chip Capture/cDNA Creation.|
|Up to 96 cells. Rate includes: capture chip and reagents, cDNA conversion reagents (Clontech SMARTer) and instrument/labor costs.||1-any||run||$1,993.53|
|Single-cell RNA-Seq Library Creation.|
|Library creation from cDNA utilizes Illumina Nextera technology, optimized for low-cost, quarter-scale reactions.||1-23
|UIC Microscope Access.||UIC Microscope Access (per 1/2 hour) is charged to all C1 captures and is used to determine capture efficiency.||1-any||1/2 hour||$17.67|
|Single-chip Capture + 3’ RNA-Seq Library Creation.||Up to 800 cells. Service includes: capture chip and all reagents for cDNA creation and 3’ RNA-Seq library creation.||1-any||run||$4,464.29|
Contact Jerry Daniel at email@example.com to schedule a capture time. Client needs to be present during scheduled capture time in 1-210 Cancer & Cardiovascular Research Building.Complete the C1 Project Initiation Form.
Bring 250 cell / uL in a minimum 20 uL media. Single-cell suspension can be submitted in any type of media. Note: Please provide extra media in case the single-cell suspension needs to be diluted.
An Excel report is emailed to client with PicoGreen concentrations with a link to images in the Imaging Center.