New Products & Technologies

Explore new genomic products and technology updates that were commercially launched in the first half of 2021. The newest product is listed first in each section. Note: This is not a comprehensive list of recently released genomic products but are products now available at the UMGC or are related to a service that we offer.

Last updated July 2021. 

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Exome Sequencing

SureSelect Human All Exon V8

In June Agilent launched its SureSelect Human All Exon V8 with a new exome design that provides comprehensive content and up-to-date coverage of protein-coding regions. The panel delivers excellent enrichment performance for more uniform coverage, as well as efficient and cost-effective exome sequencing. 

  • Coverage of protein-coding regions from RefSeq, CCDS, and GenCode.
  • Coverage of the TERT promoter and hard-to-capture exons.
  • New design to span a 35.1 Mb target region of the human genome.
  • Three versions: routine exome sequencing (Exome v8); clinical research sequencing (v8 Clinical Plus); and translational research (v8 UTR Plus).

Contact Matt Arriola at next-gen@umn.edu with questions. 

Read the Data Sheet

Next-generation Sequencing

NextSeq 2000

Now installed at the UMGC. Illumina's latest sequencer, the NextSeq 2000, leverages the latest advances in optics, instrument design, and reagent chemistry to miniaturize the volume of the sequencing reaction while increasing output and reducing the cost per run.

Using a patterned, high-output flow cell similar to those that power the NovaSeq, the P3 reagent kit for the NextSeq 2000 can generate 1.1 billion reads per run, compared to 400 million with the NextSeq 550. The result is a highly flexible and scalable benchtop system that offers the highest cluster density flow cell of any on-market NGS system to date, driving down the cost per gigabase of the sequencing run, when compared with the NextSeq 550 price/Gb.

The P2 and P3 kits will be available in four configurations: the 100-cycle kit delivers 110 Gb per run; the 200-cycle kit, 220 Gb; and the 300-cycle kit, 330 Gb. A 50-cycle P3 kit is also available for infectious disease, small RNA, and spatial transcriptomics applications.

The two UMGC NextSeq 550s will retire by the end of the summer. See our Next-gen Sequencing service page for information on our other Illumina sequencers or contact next-gen@umn.edu with questions. 

Read the Spec Sheet

RNA-Seq

Stranded Total RNA Prep, Ligation with Ribo-Zero™ Plus

TruSeq™ Stranded Total RNA provides a robust solution for whole transcriptome analyses for standard and low-quality samples. However, a relatively high input requirement, long total assay time and hands-on time, and narrow application flexibility have limited its utility in total RNA-Seq applications. 

To overcome these challenges, Illumina developed Illumina Stranded Total RNA Prep. Now available at the UMGC, this advanced solution offers streamlined, rapid ligation-based library preparation that supports low sample inputs and a wide range of RNA-Seq applications. 

To focus studies on high-value sequences, Illumina Stranded Total RNA Prep includes the Illumina Ribo-Zero Plus rRNA Depletion Kit, which provides efficient removal of ribosomal RNA from multiple species, including human, mouse, rat, and bacteria, in a single reaction.

Comparison of RNA-Seq kits available at the UMGC. 1) Illumina Stranded Total RNA Prep includes the Illumina Ribo-Zero Plus rRNA Depletion Kit, which provides efficient removal of ribosomal RNA from multiple species, including human, mouse, rat, and bacteria. 2) UMGC sample submission requirements.

Contact Next-gen with questions

Single-cell Genomics

3’ CellPlex Kit

Scale your single-cell studies with the new 10X Genomics 3′ CellPlex Kit for sample multiplexing. Reduce sample costs and enable large-scale studies or characterize rare cell types and states for Single Cell Gene Expression experiments. Contact single-cell@umn.edu for pricing. 

  • Multiplex up to 12 samples per channel to enable increased sample throughput for larger single-cell experiments and reduced cost per sample.
  • Capture up to 30,000 cells per channel to increase statistical power for analysis of rare cell populations.
  • Combine multiple samples in the same channel to help minimize batch effects and simplify data analysis.
  • Note: The 5' compatible CellPlex solution is anticipated in the first half of 2022.

Find out more 

Single Cell Low Throughput Gene Expression

Whether you are just getting started with single-cell gene expression, running pilot studies, or optimizing experimental conditions, the 10X Genomics Chromium Single Cell Gene Expression LT (low throughput) kit provides a cost-effective solution for smaller-scale projects. Researchers can generate high-quality pilot data before transitioning to full-scale studies.

  • Profiles gene expression for 100 to 1,000 single cells by barcoding mRNA at the 3’ end.
  • Combines gene expression analysis with detection of hundreds of cell surface proteins using oligonucleotide-conjugated antibodies.
  • Enables cost-effective optimization of experimental conditions and sample prep.
  • Conduct pilot studies and generate preliminary data with reduced upfront costs.

Contact the UMGC at single-cell@umn.edu to discuss single-cell pilot options. 

Getting Started Guide

Spatialomics

Visium Spatial Gene Expression for FFPE

Available as of June 2021, the Visium Spatial Gene Expression for FFPE combines the benefits of histological techniques with the massive throughput and discovery power of RNA sequencing in formalin fixed paraffin embedded (FFPE) tissue samples for unbiased gene expression. Contact spatialomics@umn.edu for more information. 

  • Spatially profile RNA expression for over 18,000 genes in human and mouse FFPE samples.
  • Unique RTL probe design for highly specific and sensitive detection of the whole transcriptome.
  • Combine with immunofluorescence for visualization of protein and gene expression or with H&E for morphological context. 
  • FFPE Tested Tissue List.

Read the Product Sheet

GeoMx Mouse Whole Transcriptome Atlas

Released in May 2021, the new GeoMX Mouse Whole Transcriptome Assay enables researchers to apply spatial transcriptomics to reveal the tissue architecture and underlying function in genetically modified mouse models. Contact spatialomics@umn.edu for more information. 

  • RNA Probe set that targets 21,000+ transcripts for mouse protein coding genes plus ERCC negative controls to profile the whole transcriptome. 
  • Excludes uninformative high expressing targets such as ribosomal subunits.
  • Includes RNA probes designed for Illumina NGS readout with the Seq Code library prep.
  • Quantity: 4 slides. Sample types include FFPE, Frozen Fresh, Fixed Frozen, and TMAs.

Read the Product Bulletin

GeoMx Human Whole Transcriptome Atlas

Launched in February 2021, the GeoMx Human Whole Transcriptome Atlas delivers the maximum amount of sensitivity and confidence in each transcript through its unique probe architecture. Researchers can explore RNA profiling in oncology, immunology, neuroscience, developmental biology, and other diverse fields. 

  • RNA Probe set that targets 18,000+ transcripts for human protein coding genes plus ERCC negative controls to profile the whole transcriptome.
  • Excludes uninformative high expressing targets such as ribosomal subunits.
  • Includes RNA probes designed for Illumina NGS readout with the Seq Code library prep.
  • Compatible with common sample types such as FFPE or fresh frozen and acrossall human tissues.
  • Whitepaper: The GeoMx Human Whole Transcriptome Atlas for the Digital Spatial Profiler: Design, Performance, and Experimental Guidelines.

Read the Product Bulletin

Stem Cell Biology

nCounter Stem Cell Characterization Panel

NanoString launched in June the nCounter Stem Cell Characterization Panel for the analysis and optimization of stem cell lines used in the development of novel therapeutics.

The 770-gene panel is available for humans and mice and was designed at NanoString with input from leading stem cell experts. The panel measures eight essential components of stem cell biology and provides a standardized assay for evaluating factors that influence and determine viability, functionality, and pluripotency.

See our nCounter service page or contact Darrell Johnson at exprn@umn.edu with questions. 

Read the Product Bulletin