DNA Methylation Library Preparation
Next-generation sequencing is a powerful means to profile DNA methylation at the single-nucleotide resolution when it is combined with bisulfite conversion of DNA either before or after library creation.
Exposure of DNA to sodium bisulfite results in the conversion of cytosine (C) – but not methylated cytosine (C*) – to uracil (U). Subsequent amplification of DNA during PCR converts U to T. Consequently, bisulfite conversion results in the change of C residues in DNA to T, leaving *C unchanged. Once such conversion has been accomplished, the original methylation state – C or C* – can be read out as the presence of a C vs T at a given CpG site.
Questions?
Contact Aaron Becker: next-gen@umn.edu.
Standard
Please inquire about RRBS library creation rates at next-gen@umn.edu. There are a large variety of other methods for the measurement of DNA methylation in DNA, including whole-genome methods, methods based on sequence capture of CpG islands, and targeted methods. Contact us for details.
Submission
How to Order
1. Please contact next-gen@umn.edu for project specifications.
2. Once project details are finalized, complete the appropriate submission form for submitting samples or submitting client-made libraries and email to next-gen@umn.edu.
Illumina Sequencing Request Form (Libraries)
Illumina Sequencing Request Form (Samples)
Shipping Instructions
Samples should be frozen and shipped on dry ice in a 96-well plate. We recommend using plate tape to seal the wells. Place the plate inside of a plastic bag prior to placing on dry ice. Please give advance notice of submission date and time so staff can be prepared to receive samples. If shipping samples from outside the University of Minnesota, ship via express shipping carrier on dry ice to the address below.
Samples can be dropped off at one of our campus locations
1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
20 Snyder Hall (St. Paul campus)
Samples can be shipped to the following address:
Please send the tracking information to next-gen@umn.edu.
UMN Genomics Center
ATTN: Nasia Mead
3510 Hopkins Place N.
Building 4 Suite W402
Oakdale, MN 55128
612-625-7736
FAQ
What is the turnaround time?
Generally, turnaround is 6-8 weeks for standard NGS projects. However, please contact next-gen@umn.edu for specific project turnaround time.