Fluidigm C1 for DNA-SEQ and ATAC-SEQ


Overview

DNA-SEQ


For DNA-Seq on individual cells, the C1 instrument carries out on-chip whole-genome amplification. Material that is harvested from the instrument can be used in downstream NGS, in combination with methods such as sequence capture to focus on exonic regions, or other methods for sequence enrichment.

scATAC-SEQ


Chromatin accessibility can be mapped, genome-wide, using the “Assay for Transposase-Accessible Chromatin” combined with Next-generation Sequencing (ATAC-Seq). This method has been protocolozed on the Fluidigm C1 as the “scATAC-Seq” method.

Questions?


Contact us at single-cell@umn.edu

Pricing

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Standard

Service Scale Unit UMN External
DNA-Seq.        
Up to 96 cells. 1-any run Inquire Inquire
scATAC-Seq.        
Up to 96 cells. 1-any run Inquire Inquire
Guidelines

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Submission

How to Order


Contact us at single-cell@umn.edu to schedule a capture time. Client needs to be present during scheduled capture time in 1-210 Cancer & Cardiovascular Research Building.

Complete the C1 Project Initiation Form.

Bring 250 cell / uL in a minimum 20 uL media. Single-cell suspension can be submitted in any type of media. Note: Please provide extra media in case the single-cell suspension needs to be diluted.

Deliverables

Captured cells.

An Excel report is emailed to client with PicoGreen concentrations with a link to images in the Imaging Center.