DNA Library Preparation
DNA-Seq methods differ in terms of the DNA fragmentation method, genome size of interest, use of PCR amplification in library creation, and the use of “mate-pair” technology. All of the protocols listed in this section utilize Illumina reagents and protocols.
TruSeq Nano
This is a “standard” NGS workflow: 1) acoustic shearing of DNA, 2) enzymatic repair/ligation of adaptors, and 3) PCR amplification.
Nextera XT
This “transposase” method does not involve physical fragmentation of gDNA, but instead uses direct incorporation of Illumina adaptors via “tagmentation”.
This workflow is tailored to small genomes, such as those of bacteria. In both Nextera and Nextera XT methods, PCR is used to amplify successful tagmented molecules.
PCR-Free
This method is designed to avoid GC-bias associated with methods such as TruSeq and Nextera.
Questions?
Contact Elyse Froehling: next-gen@umn.edu.
UMN Rates
| Illumina kit options | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Nextera XT. Quarter RXN. W/ library QC. | $42.20 | $61.34 | $108.92 |
| Turnaround time. | 10 to 15 days | 6 to 10 days | 3 to 5 days |
| Nextera XT. Quarter RXN. No library QC. | $33.38 | $48.13 | $75.79 |
| Turnaround time. | 14 to 22 days | 8 to 13 days | 4 to 7 days |
| Nextera XT. Full-scale reaction. | $80.08 | $109.69 | $175.50 |
| Turnaround time. | 10 to 15 days | 6 to 10 days | 3 to 5 days |
| DNA Prep. | $88.20 | $125.76 | $182.00 |
| Turnaround time. | 10 to 15 days | 6 to 10 days | 3 to 5 days |
| TruSeq Nano. | $100.89 | $188.74 | $224.33 |
| Turnaround time. | 13 to 18 days | 7 to 13 days | 5 to 7 days |
Notes
Tiers: Turnaround time (TAT) is in business days. TAT is for library prep only and does not factor in the TAT for sequencing or other companion services. Availability for the Priority Tier is limited; contact next-gen@umn.edu to see if this is a current option. More information about Pricing Tiers.
External Rates
| Illumina kit options | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Nextera XT. Quarter RXN. W/ library QC. | $53.84 | $75.13 | $128.01 |
| Turnaround time. | 10 to 15 days | 6 to 10 days | 3 to 5 days |
| Nextera XT. Quarter RXN. No library QC. | $42.59 | $59.00 | $89.75 |
| Turnaround time. | 14 to 22 days | 8 to 13 days | 4 to 7 days |
| Nextera XT. Full-scale reaction. | $102.17 | $135.07 | $208.22 |
| Turnaround time. | 10 to 15 days | 6 to 10 days | 3 to 5 days |
| DNA Prep. | $112.52 | $154.27 | $216.81 |
| Turnaround time. | 10 to 15 days | 6 to 10 days | 3 to 5 days |
| TruSeq Nano. | $128.69 | $226.30 | $265.93 |
| Turnaround time. | 13 to 18 days | 7 to 13 days | 5 to 7 days |
Notes
Tiers: Turnaround time (TAT) is in business days. TAT is for library prep only and does not factor in the TAT for sequencing or other companion services. Availability for the Priority Tier is limited; contact next-gen@umn.edu to see if this is a current option. More information about Pricing Tiers.
Submission
How to Order
1. Please contact next-gen@umn.edu for project specifications.
2. Once project details are finalized, complete the appropriate submission form for submitting samples or submitting client-made libraries and email to next-gen@umn.edu.
Illumina Sequencing Request Form (Libraries)
Illumina Sequencing Request Form (Samples)
Shipping Instructions
Samples should be frozen and shipped on dry ice in a 96-well plate. We recommend using plate tape to seal the wells. Place the plate inside of a plastic bag prior to placing on dry ice. Please give advance notice of submission date and time so staff can be prepared to receive samples. If shipping samples from outside the University of Minnesota, ship via express shipping carrier on dry ice to the address below.
Samples can be dropped off at one of our campus locations
- 1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
- 20 Snyder Hall (St. Paul campus)
Samples can be shipped to the following address:
Please send the tracking information to next-gen@umn.edu.
UMN Genomics Center
ATTN: Nasia Mead
3510 Hopkins Place N.
Building 4 Suite W402
Oakdale, MN 55128
612-625-7736
FAQ
What is the turnaround time?
Generally, turnaround is 6-8 weeks for standard NGS projects. However, please contact next-gen@umn.edu for specific project turnaround time.