Genotyping
The UMGC offers several different genotyping services. The choice of platform depends primarily on the number of SNPs to be genotyped, and secondarily on the number of samples.
Our Uniplex genotyping service is ideal for projects assaying a smaller number (1-6 SNPs). The service uses allele-specific Taqman genotyping primer-probe sets, purchased as pre-designed commercial assays from Applied Biosystems (ThermoFisher). One aliquot of primer-probe cocktail is generally adequate for genotyping several hundred samples.
When a project demands high-density or genome-wide coverage, Illumina’s Infinium Beadchips provide the answer. Capable of genotyping between 3072 and over 5 million SNPs, Illumina Infinium offer the most comprehensive analysis short of sequencing.
| # of SNPs of interest | Minimum # of samples* | Best Platform |
|---|---|---|
| 1-6 SNPs | 96, 192 or 384 | Uniplex |
| 3072 SNPs | 1,152 | Illumina Infinium |
*You can submit fewer than the minimum number of samples listed, but you will still be charged for the number of samples listed. The precise minimum for the Uniplex platform is dependent on the number of SNPs in question. Please inquire for details.