RNA Sequencing (RNA-Seq) has become the gold standard method for genome-wide gene expression analysis in different biological conditions, as well as the detection of splice variants, transcriptome analysis, and the discovery non-coding RNAs.
The UMGC provides several different library kit options for RNA-Seq library creation to accommodate varying sample types, including kits for low-input and partially degraded samples, as well as options for polyA enrichment or ribosomal reduction. We offer a complete RNA-Seq workflow starting from RNA extraction through RNA QC analysis, and in partnership with MSI, analysis of RNA-Seq data through streamlined pipelines.
Please see our PacBio service to sequence full-length transcripts up to 10kb or longer and our Single-cell service for gene expression profiling of single cells.
Library Prep Options
Comparison of RNA-Seq kits available at the UMGC. 1) Illumina Stranded Total RNA Prep includes the Illumina Ribo-Zero Plus rRNA Depletion Kit, which provides efficient removal of ribosomal RNA from multiple species, including human, mouse, rat, and bacteria. 2) UMGC sample submission requirements.
Questions?
For questions or a quote, please contact Elyse Froehling at [email protected]. If your preferred RNA-Seq kit is not listed among our standard offerings, we can accommodate non-standard protocols when possible. Please reach out to discuss your project needs.
UMN Rates
| Watchmaker kit options | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Stranded mRNA. | $61.38 | $88.01 | $160.97 |
| Turnaround time. | 11 - 16 days | 6 - 10 days | 4 - 5 days |
| Stranded RNA. | $79.10 | $107.15 | $190.75 |
| Turnaround time. | 11 - 16 days | 6 - 10 days | 4 - 5 days |
| Illumina kit options | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| mRNA. TruSeq Stranded. | $128.59 | $168.28 | $289.41 |
| Turnaround time. | 10 - 13 days | 5 - 9 days | 3 - 4 days |
| Total RNA. Stranded. Lig w RZ Plus. | $158.01 | $195.48 | $311.38 |
| Turnaround time. | 10 - 13 days | 5 - 9 days | 3 - 4 days |
| Takara kit options | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| SMART-Seq Total RNA Pico ZapR-M. | $109.85 | $145.32 | $231.23 |
| Turnaround time. | 10 - 13 days | 5 - 9 days | 3 - 4 days |
| Small RNA. | $157.20 | $205.68 | $328.12 |
| Turnaround time. | 10 - 13 days | 5 - 9 days | 3 - 4 days |
Notes
Library Creation: Standalone library creation rate. Please see our sequencing rates for your own dedicated lane and read length and depth. Standard sequencing queue turnaround applies. RNA sample QC (Ribogreen + RNA sizing) is additional.
Tiers: Turnaround time (TAT) is in business days. TAT is for library prep only and does not factor in the TAT for sequencing or other companion services. Availability for the Priority Tier is limited; contact [email protected] to see if this is a current option. More information about Pricing Tiers.
External Rates
| Watchmaker kit options | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Stranded mRNA. | $70.85 | $98.06 | $173.62 |
| Turnaround time. | 11 - 16 days | 6 - 10 days | 4 - 5 days |
| Stranded RNA. | $90.86 | $128.40 | $205.69 |
| Turnaround time. | 11 - 16 days | 6 - 10 days | 4 - 5 days |
| Illumina kit options | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| mRNA. TruSeq Stranded. | $158.06 | $202.16 | $336.87 |
| Turnaround time. | 10 - 13 days | 5 - 9 days | 3 - 4 days |
| Total RNA. Stranded. Lig w RZ Plus. | $194.24 | $235.87 | $364.83 |
| Turnaround time. | 10 - 13 days | 5 - 9 days | 3 - 4 days |
| Takara kit options | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| SMART-Seq Total RNA Pico ZapR-M. | $135.03 | $174.45 | $269.94 |
| Turnaround time. | 10 - 13 days | 5 - 9 days | 3 - 4 days |
| Small RNA. | $193.25 | $247.25 | $383.23 |
| Turnaround time. | 10 - 13 days | 5 - 9 days | 3 - 4 days |
Notes
Library Creation: Standalone library creation rate. Please see our sequencing rates for your own dedicated lane and read length and depth. Standard sequencing queue turnaround applies. RNA sample QC (Ribogreen + RNA sizing) is additional.
Tiers: Turnaround time (TAT) is in business days. TAT is for library prep only and does not factor in the TAT for sequencing or other companion services. Availability for the Priority Tier is limited; contact [email protected] to see if this is a current option. More information about Pricing Tiers.
Contact us with your for project specifications. Once project details are finalized, complete the appropriate submission form for submitting samples or submitting client-made libraries and email it to [email protected].
Campus drop-off locations:
- 1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
- 20 Snyder Hall (St. Paul campus)
Shipping information:
Samples should be frozen and shipped on dry ice in a 96-well plate. We recommend using plate tape to seal the wells. Place the plate inside of a plastic bag prior to placing on dry ice. Please give advance notice of submission date and time so staff can be prepared to receive samples. If shipping samples from outside the University of Minnesota, ship via express shipping carrier on dry ice to the address below.
UMN Genomics Center
ATTN: NGS Staff
3510 Hopkins Place N.
Building 4 Suite W402
Oakdale, MN 55128
612-625-7736
Please send the tracking information to [email protected].