NGS Library Quality Control
The UMGC welcomes client-submitted NGS libraries but requires UMGC quality control assessment prior to sequencing. Client NGS library QC includes three bundled assays:
- PicoGreen quantification
- Quantitative capillary electrophoretic sizing (Agilent)
- Functional quantification (KAPA Biosystems Q-PCR)
Questions
Please contact [email protected] for any questions about library QC.
UMN Rates
| Service | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| KAPA Library QC. | $90.76 | $129.75 | $183.74 |
| Turnaround time. | 7 - 10 days | 4 - 6 days | 3 - 4 days |
Notes
1. Library QC is required for libraries prepared outside the UMGC. This rate includes KAPA QC, PicoGreen, and Agilent sizing.
Tiers: Turnaround time (TAT) is in business days. Availability for the Priority Tier is limited; contact [email protected] to see if this is a current option. More information about Pricing Tiers.
External Rates
| Service | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| KAPA Library QC. | $112.23 | $155.52 | $215.53 |
| Turnaround time. | 7 - 10 days | 4 - 6 days | 3 - 4 days |
Notes
1. Library QC is required for libraries prepared outside the UMGC. This rate includes KAPA QC, PicoGreen, and Agilent sizing.
Tiers: Turnaround time (TAT) is in business days. Availability for the Priority Tier is limited; contact [email protected] to see if this is a current option. More information about Pricing Tiers.
Submission
How to Order
For all direct sequencing submissions of NGS libraries created outside the UMGC, please contact [email protected].
University researchers submitting NGS libraries for QC only please print and submit the completed submission form with your samples.
Submit 10 ul of sample with a concentration greater than 2 nM per sample in a 96-well plate (fill plate starting with A1-H1, A2-H2, etc.).
Forms
Deliverables
A QC report is emailed to client including library concentration (both ng/ul and nM) and size (Agilent).