NGS Library Quality Control


Overview

The UMGC welcomes client-submitted NGS libraries but requires UMGC quality control assessment prior to sequencing. Client NGS library QC includes three bundled assays:

  1. PicoGreen quantification
  2. Quantitative capillary electrophoretic sizing (Agilent)
  3. Functional quantification (KAPA Biosystems Q-PCR)

Questions


Please contact umgc-qc@umn.edu for any questions about library QC.

Pricing

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Standard

Service Scale Unit UMN External
KAPA Library QC.        
Library QC is required for libraries prepared outside the UMGC. This rate includes KAPA QC, PicoGreen, and Agilent sizing. 1-any sample $61.63 $71.15
Guidelines

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Submission

How to Order


For all direct sequencing submissions of NGS libraries created outside the UMGC, please contact next-gen@umn.edu.

University researchers submitting NGS libraries for QC only please print and submit the completed submission form with your samples.

Submit 10 ul of sample with a concentration greater than 2 nM per sample in a 96-well plate (fill plate starting with A1-H1, A2-H2, etc.).

Forms


Deliverables

A QC report is emailed to client including library concentration (both ng/ul and nM) and size (Agilent).

FAQ

What is the turnaround time?


The UMGC guarantees a turnaround time of 7 days.