NGS Library Quality Control
The UMGC welcomes client-submitted NGS libraries but requires UMGC quality control assessment prior to sequencing. Client NGS library QC includes three bundled assays:
- PicoGreen quantification
- Quantitative capillary electrophoretic sizing (Agilent)
- Functional quantification (KAPA Biosystems Q-PCR)
Questions
Please contact umgc-qc@umn.edu for any questions about library QC.
Standard
| Service | Scale | Unit | UMN | External |
|---|---|---|---|---|
| KAPA Library QC. | ||||
| Library QC is required for libraries prepared outside the UMGC. This rate includes KAPA QC, PicoGreen, and Agilent sizing. | 1-any | sample | $61.63 | $71.15 |
Submission
How to Order
For all direct sequencing submissions of NGS libraries created outside the UMGC, please contact next-gen@umn.edu.
University researchers submitting NGS libraries for QC only please print and submit the completed submission form with your samples.
Submit 10 ul of sample with a concentration greater than 2 nM per sample in a 96-well plate (fill plate starting with A1-H1, A2-H2, etc.).
Forms
Contact umgc-qc@umn.edu for a submission form.
Deliverables
A QC report is emailed to client including library concentration (both ng/ul and nM) and size (Agilent).
FAQ
What is the turnaround time?
The UMGC guarantees a turnaround time of 7 days.