DNA/RNA Sizing
Sample integrity can be assessed via automated electrophoresis. The success of downstream processing is determined by size and integrity of the sample. The UMGC uses two different platforms to assess size and quality of RNA and DNA samples: 1) Agilent 2100 Bioanalyzer and 2) Agilent 4200 TapeStation. Both instruments provide high-throughput, sensitive, and high-resolution results compared to the running of denaturing agarose gels.
Questions
For questions regarding our DNA and RNA sizing service, please contact our QC team at umgc-qc@umn.edu.
UMN Rates
| Service | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| DNA. Agilent TapeStation/Bioanalyzer. | $11.98 | $18.11 | $50.33 |
| Turnaround time. | 5 to 10 days | 1 to 4 days | 0 to 1 days |
| RNA. Agilent TapeStation/Bioanalyzer. | $10.60 | $15.46 | $26.03 |
| Turnaround time. | 5 to 10 days | 2 to 4 days | 1 to 2 days |
| Femto Pulse HMW Analysis.1 | $155.16 | $182.59 | $246.60 |
| Turnaround time. | 5 to 10 days | 2 to 4 days | 1 to 2 days |
Notes
1. Femto Pulse rate is per run. Each run can hold up to 11 samples or libraries.
Tiers: Turnaround time (TAT) is in business days. Availability for the Priority Tier is limited; contact umgc-qc@umn.edu to see if this is a current option. More information about Pricing Tiers.
External Rates
| Service | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| DNA. Agilent TapeStation/Bioanalyzer. | $15.28 | $22.10 | $57.89 |
| Turnaround time. | 5 to 10 days | 1 to 4 days | 0 to 1 days |
| RNA. Agilent TapeStation/Bioanalyzer. | $13.53 | $18.92 | $30.66 |
| Turnaround time. | 5 to 10 days | 2 to 4 days | 1 to 2 days |
| Femto Pulse HMW Analysis.1 | $197.93 | $228.61 | $299.73 |
| Turnaround time. | 5 to 10 days | 2 to 4 days | 1 to 2 days |
Notes
1. Femto Pulse rate is per run. Each run can hold up to 11 samples or libraries.
Tiers: Turnaround time (TAT) is in business days. Availability for the Priority Tier is limited; contact umgc-qc@umn.edu to see if this is a current option. More information about Pricing Tiers.
Submission
How to Order
Print and submit the completed submission form with your samples.
For Agilent DNA sizing, submit 4 ul of sample in a 96-well plate (fill plate starting with A1-H1, A2-H2, etc.).
Note: Capillary electrophoresis is sensitive to ionic contaminants. Hence, higher salt concentrations can interfere with performance of samples in molecular sizing. We recommend cleaning up samples for Agilent Qualitative Analysis. Please elute in water or 10mM Tris-HCL, pH 8.5 for best results.
Forms
Samples can be dropped off at one of our laboratory locations.
Please make sure samples are submitted directly to a UMGC staff member so that the samples are stored appropriately. DNA samples can be placed in the indicated freezer.
- 1-210 Cancer & Cardiovascular Research Building (Minneapolis campus).
- 20 Snyder Hall (St. Paul campus) – self-serve drop-off room.
Deliverables
Electropherogram and gel pseudo images are emailed to client.
FAQ
What is the turnaround time?
The UMGC guarantees a turnaround time of 3 days. Often results are sent out within 24 hours of sample submission.
Agilent Assays
| Assay | Concentration (ng/ul) | Size Range (bp) |
|---|---|---|
| DNA 1000 | 0.1 – 50 | 25 – 1000 |
| High Sensitivity DNA | 0.005 – 1 | 35 – 7000 |
| High Sensitivity D5000 | 0.01 – 1 | 100 – 5000 |
| DNA 12000 | 0.5 – 50 | 100 – 12000 |
| Genomic DNA | 10 – 100 | 200 – >60,000 |