The UMGC provides digital PCR through the QX200 Droplet Digital PCR (ddPCR) system produced by Bio-Rad. This technology offers flexible digital PCR chemistry that can use Taqman hydrolysis probes or EvaGreen assays for high-precision absolute quantification of nucleic acid targets without the need for a standard curve. Digital PCR is ideal for measurements of copy number variation, rare sequence detection, gene expression, target DNA/RNA, and microbial quantification.
We offer two ddPCR service options to serve our University of Minnesota clients. Our current service options are client operated to give users ultimate flexibility with experimental design and project turnaround.
Clients purchase their own Bio-Rad reagents and consumables, including the EvaGreen Supermix or the Supermix for Probes, and use a dedicated ddPCR workstation to complete the following QX200 workflow:
- Reaction set-up
- Droplet generation
- PCR amplification
- Droplet reading
- ddPCR analysis
Please note, one-time instrument training is required for all new users before using the ddPCR workstation.
Questions?
Please contact Darrell Johnson at [email protected] for further details on the QX200 ddPCR system.
UMN Rates
| Library Creation | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Full-service. One-step. AutoDG per 32 rxns. | $181.35 | $284.76 | $358.02 |
| Turnaround time. | 4 - 5 days | 2 - 3 days | 1 - 2 days |
| Full-service. One-step. Per sample. | $14.11 | $17.94 | $30.58 |
| Turnaround time. | 4 - 5 days | 2 - 3 days | 1 - 2 days |
| Full-service. Evagreen. Per Sample. | $8.76 | $11.36 | $21.95 |
| Turnaround time. | 4 - 5 days | 2 - 3 days | 1 - 2 days |
| Hybrid-service (CoLab). AutoDG per 32 rxns. | $142.30 | N/A | N/A |
External Rates
| Library Creation | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Full-service. One-step. AutoDG per 32 rxns. | $233.78 | $337.90 | $419.16 |
| Turnaround time. | 4 - 5 days | 2 - 3 days | 1 - 2 days |
| Full-service. One-step. Per sample. | $17.35 | $21.61 | $35.65 |
| Turnaround time. | 4 - 5 days | 2 - 3 days | 1 - 2 days |
| Full-service. Evagreen. Per Sample. | $10.77 | $13.66 | $25.43 |
| Turnaround time. | 4 - 5 days | 2 - 3 days | 1 - 2 days |
| Hybrid-service (CoLab). AutoDG per 32 rxns. | $184.86 | N/A | N/A |
Notes
1. Plates and seals are separate charges if not provided by the researcher.
2. Tiers: Turnaround time (TAT) is in business days. Availability for the Priority Tier is limited; contact us to see if this is a current option.
Contact [email protected] to get started.
Locations for training and the ddPCR workstation:
4Front (Off-campus)
3510 Hopkins Place North
BLDG 4 - Suite W402
Oakdale, MN 55128
Lead: Darrell Johnson
Phone: 612-625-7736
Operating Hours: 9:00 AM – 6:00 PM Monday – Friday
Deliverables:
The data is retrieved by client after their droplet reading via USB Flash Drive, Dropbox, Google Drive, email, etc. If time permits, clients have the option of using the QuantaSoft software at the ddPCR workstation. Alternatively, QuantaSoft is available by download after a Bio-Rad account is created and clients can analyze data on their lab PC.
For client who elect for the UMGC Read-only service, once your droplets have finished reading, we will send you the entire data file via google drive or the .csv file by email. Clients can download the QuantaSoft software for in-house analysis of their droplet data.