Illumina MethylationEPIC


Overview

The Illumina Infinium MethylationEPIC BeadChip is based on the Illumina Infinium genotyping platform, and queries individual CpG sites across the human genome by measuring the C/T ratio following bisulfite conversion of DNA, thereby permitting the % methylation of each CpG site to be ascertained. This 850,000-feature array is an update to Illumina’s popular HumanMethylation450 methylation platform with the addition of Enhanced and Promoter Informed Content. This beadchip contains > 90% of the original Infinium Methylation450 BeadChip content plus an additional 350,000 CpGs in enhancer regions. Content on the BeadChip was selected by a global consortium of epigenomics experts and covers:

  • CpG islands
  • RefSeq genes
  • ENCODE open chromatin
  • ENCODE transcription factor binding sites
  • FANTOM5 enhancers

As Certified Service Providers for the Illumina Infinium arrays, we undergo annual certification to guarantee our clients receive reliable methylation values for each CpG locus using the most up-to-date workflows. Our EPIC array rates, found under the pricing tab, include charges for bisulfite conversion + array processing. There is an additional charge for shipping the arrays.

Click here for more details on the MethylationEPIC array’s design and performance. Below are links to publications describing the validation of the platform.

https://genomebiology.biomedcentral.com/articles/10.1186/s13059-016-1066-1

https://clinicalepigeneticsjournal.biomedcentral.com/articles/10.1186/s13148-017-0333-7

Questions?


Contact Jerry Daniel: genotype@umn.edu

Certification


The UMGC is a certified service provider for the Illumina Infinium.

Pricing

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Standard

Service Scale Unit UMN External
Illumina Infinium MethylationEPIC
Low scale. 8-16 sample $236.17 $282.48
Standard scale. 17-96 sample $203.21 $239.47
High scale. 97-384 sample $198.63 $238.63
Very high scale. 385-any sample $194.50 $229.41

Notes

Pricing costs include the bisulfite conversion, EPIC arrays, and reagents.

Guidelines

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Submission

How to Order


Complete the DNA Plate Submission Form.

For University researchers, submit the completed form through the online ordering system by creating an order for “Genotyping, Epigenomics and CNV Analysis” by clicking on the “SNP and Sample Submission Form”. For researchers outside the University, submit the completed form to genotype@umn.edu.

Researchers should Submit a minimum of 500 ng of DNA per sample at 25 ng/ul in a 96-well plate in a COLUMN-WISE fashion RANDOMIZING samples and keeping two unique blank wells. Please review our MethylationEPIC Sample Submission Form Guidelines.

Samples can be dropped-off at one of our laboratory locations.

1-202 Nils Hasselmo Hall (Minneapolis campus)
1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
20 Snyder Hall (St. Paul campus)

If shipping samples, the following address should be used.

Please send the tracking information to genotype@umn.edu.

University of Minnesota Genomics Center
Genotyping
312 Church St SE, 1-202 Nils Hasselmo Hall
Minneapolis, MN 55455
612-624-3177

Deliverables

Client is emailed the GenomeStudio project file as well as data in Excel.