Quantification of nucleic acids can be achieved via UV spectroscopy or via the use of fluorometry (in combination with DNA (PicoGreen) or RNA (RiboGreen)-binding dyes).
The NanoDrop instrument for UV quantification consumes only 1-2 ul of material, and provides a measure of DNA purity (260/280 and 260/230 ratios), but is not able to accurately quantify impure nucleic acids or DNA or RNA at concentrations below ≈ 10 ng/ul.
PicoGreen and RiboGreen quantification are therefore the gold standards for sensitive and specific quantification of DNA and RNA, respectively.
Questions
Please contact our QC team at [email protected] for more information and questions regarding quantification.
UMN Rates
| Service | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| NanoDrop Quantification. | $2.50 | $5.07 | $12.93 |
| Turnaround time. | 4 - 6 days | 2 - 4 days | 0 - 1 day |
| PicoGreen DNA Quantification. | $2.45 | $9.77 | N/A |
| Turnaround time. | 5 - 7 days | 2 - 4 days | N/A |
| RiboGreen RNA Quantification. | $2.53 | $9.87 | N/A |
| Turnaround time. | 5 - 8 days | 2 - 4 days | N/A |
| Qubit dsDNA BR/HS Quantification. | $8.75 | $10.36 | $15.28 |
| Turnaround time. | 1 - 3 days | 1 - 2 days | 1 day |
| Qubit RNA BR/HS Quantification. | $7.31 | $9.13 | $14.41 |
| Turnaround time. | 3 - 7 days | 3 - 4 days | 1 - 2 days |
| QC DNA. Qubit Quant and Agilent Sizing. | $21.32 | $27.48 | $53.46 |
| Turnaround time. | 3 - 6 days | 2 - 4 days | 2 days |
Notes:
Tiers: Turnaround time (TAT) is in business days. Availability for the Priority Tier is limited; contact [email protected] to see if this is a current option. More information about Pricing Tiers.
External Rates
| Service | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| NanoDrop Quantification. | $3.07 | $5.93 | $14.66 |
| Turnaround time. | 4 - 6 days | 2 - 4 days | 0 - 1 day |
| PicoGreen DNA Quantification. | $3.02 | $11.15 | N/A |
| Turnaround time. | 5 - 7 days | 2 - 4 days | N/A |
| RiboGreen RNA Quantification. | $3.11 | $11.26 | N/A |
| Turnaround time. | 5 - 8 days | 2 - 4 days | N/A |
| Qubit dsDNA BR/HS Quantification. | $10.76 | $12.55 | $18.02 |
| Turnaround time. | 1 - 3 days | 1 - 2 days | 1 day |
| Qubit RNA BR/HS Quantification. | $8.99 | $11.01 | $16.87 |
| Turnaround time. | 3 - 7 days | 3 - 4 days | 1 - 2 days |
| QC DNA. Qubit Quant and Agilent Sizing. | $26.22 | $33.06 | $61.94 |
| Turnaround time. | 3 - 6 days | 2 - 4 days | 2 days |
Notes:
Tiers: Turnaround time (TAT) is in business days. Availability for the Priority Tier is limited; contact [email protected] to see if this is a current option. More information about Pricing Tiers.
Submission
How to order
Print and submit the completed submission form with your samples.
Please submit 4 ul of sample per assay in a 96-well plate (fill plate starting with A1-H1, A2-H2, etc.). Example: if requesting both NanoDrop and PicoGreen, please submit 8 ul of sample.
For NanoDrop submissions, please also provide 2 ul/sample blanking solution if other than water.
Forms
Samples can be dropped off at one of our laboratory locations.
For RNA samples, please make sure samples are submitted directly to a UMGC staff member so that the samples are stored appropriately. DNA samples can be placed in the indicated freezer.
- 1-210 Cancer & Cardiovascular Research Building (Minneapolis campus).
- 20 Snyder Hall (St. Paul campus) – self-serve drop-off room.
Deliverables
An Excel report is emailed to client with sample IDs and concentrations.