Quantification of DNA or RNA


Overview

Quantification of nucleic acids can be achieved via UV spectroscopy or via the use of fluorometry (in combination with DNA (PicoGreen) or RNA (RiboGreen)-binding dyes).

The NanoDrop instrument for UV quantification consumes only 1-2 ul of material, and provides a measure of DNA purity (260/280 and 260/230 ratios), but is not able to accurately quantify impure nucleic acids or DNA or RNA at concentrations below ≈ 10 ng/ul.

PicoGreen and RiboGreen quantification are therefore the gold standards for sensitive and specific quantification of DNA and RNA, respectively.

Questions

Please contact our QC team at umgc-qc@umn.edu for more information and questions regarding quantification.

Pricing

Expand all

Standard

Service Scale Unit UMN External
NanoDrop Quantification. 1-any sample $0.36 $0.41
PicoGreen DNA Quantification.        
Standard scale. 1-384 sample $2.30 $2.96
High scale. 385-any sample $1.87 $2.12
RiboGreen RNA Quantification. 1-any sample $4.60 $5.29

 

Guidelines

Expand all

Submission

NanoDrop Quantifiation, PicoGreen RNA Quantification, and RiboGreen RNA Quantification


Print and submit the completed submission form with your samples.

Please submit 4 ul of sample per assay in a 96-well plate (fill plate starting with A1-H1, A2-H2, etc.). Example: if requesting both NanoDrop and PicoGreen, please submit 8 ul of sample.

For NanoDrop submissions, please also provide 2 ul/sample blanking solution if other than water.

Forms


QC Submission Form – UMN researchers

QC Submission Form – External

Samples can be dropped off at one of our laboratory locations.


For RNA samples, please make sure samples are submitted directly to a UMGC staff member so that the samples are stored appropriately. DNA samples can be placed in the indicated freezer.

1-202 Nils Hasselmo Hall (Minneapolis campus) – Freezer #1.

1-210 Cancer & Cardiovascular Research Building (Minneapolis campus) small freezer in the lobby.

28 Snyder Hall (St. Paul campus) – please drop off directly with UMGC staff. We have a fridge, not a freezer in Snyder 20.

Deliverables

An Excel report is emailed to client with sample IDs and concentrations.

FAQ

What is the turnaround time?

The UMGC guarantees a turnaround time of 3 days. Often results are sent out within 24 hours of sample submission.