Quantification of DNA or RNA


Overview

Quantification of nucleic acids can be achieved via UV spectroscopy or via the use of fluorometry (in combination with DNA (PicoGreen) or RNA (RiboGreen)-binding dyes).

The NanoDrop instrument for UV quantification consumes only 1-2 ul of material, and provides a measure of DNA purity (260/280 and 260/230 ratios), but is not able to accurately quantify impure nucleic acids or DNA or RNA at concentrations below ≈ 10 ng/ul.

PicoGreen and RiboGreen quantification are therefore the gold standards for sensitive and specific quantification of DNA and RNA, respectively.

Questions

Please contact our QC team at [email protected] for more information and questions regarding quantification.

Pricing

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UMN Rates

ServiceSavings TierSpeed TierPriority Tier
NanoDrop Quantification.$2.43$4.88$12.31
Turnaround time.4 - 6 days2 - 4 days0 - 1 day
PicoGreen DNA Quantification.$2.43$9.37N/A
Turnaround time.5 - 7 days2 - 4 daysN/A
RiboGreen RNA Quantification.$2.50$9.46N/A
Turnaround time.5 - 8 days2 - 4 daysN/A


Notes: 
Tiers: Turnaround time (TAT) is in business days. Availability for the Priority Tier is limited; contact [email protected] to see if this is a current option. More information about Pricing Tiers

External Rates

ServiceSavings TierSpeed TierPriority Tier
NanoDrop Quantification.$3.01$5.73$13.98
Turnaround time.4 - 6 days2 - 4 days0 - 1 day
PicoGreen DNA Quantification.$3.01$10.72N/A
Turnaround time.5 - 7 days2 - 4 daysN/A
RiboGreen RNA Quantification.$3.09$10.83N/A
Turnaround time.5 - 8 days2 - 4 daysN/A


Notes: 
Tiers: Turnaround time (TAT) is in business days. Availability for the Priority Tier is limited; contact [email protected] to see if this is a current option. More information about Pricing Tiers

Guidelines

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Submission

NanoDrop Quantifiation, PicoGreen RNA Quantification, and RiboGreen RNA Quantification


Print and submit the completed submission form with your samples.

Please submit 4 ul of sample per assay in a 96-well plate (fill plate starting with A1-H1, A2-H2, etc.). Example: if requesting both NanoDrop and PicoGreen, please submit 8 ul of sample.

For NanoDrop submissions, please also provide 2 ul/sample blanking solution if other than water.

Forms


QC – UMN Submission Form

QC – CoLab Submission Form

QC – External Submission Form

Samples can be dropped off at one of our laboratory locations.


For RNA samples, please make sure samples are submitted directly to a UMGC staff member so that the samples are stored appropriately. DNA samples can be placed in the indicated freezer.

  • 1-210 Cancer & Cardiovascular Research Building (Minneapolis campus).
  • 20 Snyder Hall (St. Paul campus) – self-serve drop-off room.

Deliverables

An Excel report is emailed to client with sample IDs and concentrations.