As part of our mission to diversify our NGS offerings, the UMGC has expanded our single-cell solutions to include the Evercode split pool combinatorial barcoding technology by Parse Biosciences. By converting each cell or nucleus into an individual reaction compartment, Parse Evercode enables researchers to profile cells at various levels of scale and then tailor to suit their experiment needs.
- Scalability — up to 96 unique biological samples and 1,000,000 cells or nuclei in a single experiment with low multiplet rates.
- Workflow flexibility — by separating the sample extraction from downstream library preparation, researchers can run fixed cells or nuclei collected on different dates or at different locations together in a single experiment.
- Fixation of cells/nuclei — capture transcriptomic information immediately after sample collection with a rapid fixation protocol.
To streamline high-throughput studies, UMGC now integrates automated barcoding using an Integra liquid-handling platform, ensuring consistent reagent handling and greater reproducibility across experiments.
Library Preparation Options
The UMGC offers library preparation services beyond the Parse Evercode whole transcriptomics to include the TCR and Gene Capture immune profiling and enrichment products.
Alternatively, since Parse Evercode is an instrument-free workflow, researchers can barcode and prepare libraries from within their labs and submit libraries to the UMGC for QC and NGS sequencing.
Start a Project
Whether a researcher is new to single-cell sequencing or one of our experienced users, we can help determine which single-cell technology will best meet your experimental goals. The UMGC requests that researchers contact us at [email protected] for consultation on all incoming single-cell projects.
UMN Rates
| Whole Transcriptome | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Cell count and plate loading. Per sample. | $40.33 | $60.37 | $66.70 |
| Turnaround time. | 3 - 4 days | 1 - 2 days | 1 day |
| Barcoding. Excludes reagents. Per kit. | $1,295.23 | $1,798.49 | $2,354.75 |
| Turnaround time. | 6 - 8 days | 3 - 5 days | 1 - 2 days |
| cDNA capture and amplification. Per library. | $73.03 | $118.38 | $169.67 |
| Turnaround time. | 8 - 14 days | 3 - 8 days | 2 - 3 days |
| Sublibrary generation. Per library. | $111.45 | $165.37 | $240.91 |
| Turnaround time. | 7 - 12 days | 4 - 7 days | 3 days |
| Immune Profiling | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Sublibrary and BCR library prep. | $246.50 | $359.78 | $816.46 |
| Turnaround time. | 8 - 15 days | 5 - 9 days | 4 days |
| Sublibrary and TCR library prep. | $149.73 | $187.69 | $349.29 |
| Turnaround time. | 5 - 12 days | 4 - 7 days | 3 days |
Notes
Tiers: Turnaround time (TAT) is in business days. TAT is for the above-stated services and does not factor in the TAT for sequencing or other companion services. Availability for the Priority Tier is limited.
External Rates
| Whole Transcriptome | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Cell count and plate loading. Per sample. | $45.52 | $71.87 | $71.89 |
| Turnaround time. | 3 - 4 days | 1 - 2 days | 1 day |
| Barcoding. Excludes reagents. Per kit. | $1,462.06 | $2,152.44 | $2,521.58 |
| Turnaround time. | 6 - 8 days | 3 - 5 days | 1 - 2 days |
| cDNA capture and amplification. Per library. | $84.66 | $140.20 | $184.92 |
| Turnaround time. | 8 - 14 days | 3 - 8 days | 2 - 3 days |
| Sublibrary generation. Per library. | $127.97 | $196.96 | $261.01 |
| Turnaround time. | 7 - 12 days | 4 - 7 days | 3 days |
| Immune Profiling | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Sublibrary and BCR library prep. | $282.58 | $428.91 | $859.70 |
| Turnaround time. | 8 - 15 days | 5 - 9 days | 4 days |
| Sublibrary and TCR library prep. | $171.17 | $226.21 | $374.31 |
| Turnaround time. | 5 - 12 days | 4 - 7 days | 3 days |
Notes
Tiers: Turnaround time (TAT) is in business days. TAT is for the above-stated services and does not factor in the TAT for sequencing or other companion services. Availability for the Priority Tier is limited.
A planning meeting is needed to start a single-cell project to review your experimental goals, provide guidance on experimental design, and go over project logistics like turnaround estimates, scheduling, suspension buffer, and cell concentration requirements (varies based on the assay).
When you are ready to submit, please complete the Sample Submission Form and email it to [email protected].