For whole-genome studies requiring ultra-deep sequencing, de novo assemblies, custom analyses, non-standard experimental designs, or accelerated timelines, library preparation and next-generation sequencing are purchased separately.
In this flexible model, researchers select an appropriate library preparation method and choose their preferred sequencing output, such as a flow cell or a UG 100 wafer. We can assist with the right library prep strategy, taking into account factors such as the DNA fragmentation method, genome size, and PCR amplification during library creation.
- Select a library preparation method (see the pricing tab).
- Purchase next-generation sequencing.
- Choose a guaranteed turnaround time on the AVITI (UG 100 timelines provided at the time of quote).
However, for most projects, our per-sample whole-genome sequencing service will be the most cost-effective approach. This is an end-to-end service that includes sample quality control, library preparation, sequencing, primary data QC, and, for human and mouse projects, variant calling (VCFs) and summary reports.
Questions?
Contact Dan Bade: [email protected].
UMN Rates
| Ultima Genomics Library Prep | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| PCR-free whole genome.1 | $46.60 | $63.73 | $104.33 |
| Turnaround time. | 11 - 16 days | 6 - 10 days | 4 - 5 days |
| Library conversion.2 | $40.79 | $52.99 | $85.73 |
| Turnaround time. | 3 - 6 days | 1 - 3 days | 1 - 2 days |
| Twist Library Prep | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Twist FlexPrep. | $14.11 | $18.69 | $27.21 |
| Turnaround time. | 10 - 15 days | 5 - 9 days | 2 - 4 days |
| Illumina Library Prep | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Nextera XT. 1/4 RXN. With QC. | $45.82 | $66.14 | $118.15 |
| Turnaround time. | 8 - 11 days | 4 - 7 days | 2 - 3 days |
| Nextera XT. 1/4 RXN. No QC. | $36.07 | $51.50 | $81.23 |
| Turnaround time. | 11 - 17 days | 6 - 10 days | 3 - 5 days |
| Nextera XT. Full-scale reaction. | Inquire | Inquire | Inquire |
| Turnaround time. | 8 - 11 days | 4 - 7 days | 2 - 3 days |
| DNA Prep. | $90.35 | $114.37 | $175.04 |
| Turnaround time. | 8 - 11 days | 4 - 7 days | 2 - 3 days |
Notes
1. Contact us for other applications (i.e., single-cell, ppmSeq, ultra-deep metagenomic and metatranscriptomic sequencing, etc).
2. Conversion of non-Ultima libraries for UG 100 compatibility.
Tiers: Turnaround time (TAT) is in business days. TAT is for sequencing only and does not factor in the TAT for library prep or other companion services. Availability for the Priority Tier is limited; contact [email protected] to see if this is a current option. More information about Pricing Tiers.
External Rates
| Ultima Genomics Library Prep | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| PCR-free whole genome.1 | $61.66 | $82.24 | $129.48 |
| Turnaround time. | 11 - 16 days | 6 - 10 days | 4 - 5 days |
| Library conversion.2 | $48.19 | $63.71 | $96.71 |
| Turnaround time. | 3 - 6 days | 1 - 3 days | 1 - 2 days |
| Twist Library Prep | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Twist FlexPrep. | $19.21 | $24.81 | $35.04 |
| Turnaround time. | 10 - 15 days | 5 - 9 days | 2 - 4 days |
| Illumina Library Prep | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Nextera XT. 1/4 RXN. With QC. | $56.37 | $78.92 | $136.74 |
| Turnaround time. | 8 - 11 days | 4 - 7 days | 2 - 3 days |
| Nextera XT. 1/4 RXN. No QC. | $44.38 | $61.51 | $94.58 |
| Turnaround time. | 11 - 17 days | 6 - 10 days | 3 - 5 days |
| Nextera XT. Full-scale reaction. | Inquire | Inquire | Inquire |
| Turnaround time. | 8 - 11 days | 4 - 7 days | 2 - 3 days |
| DNA Prep. | $111.10 | $137.76 | $205.19 |
| Turnaround time. | 8 - 11 days | 4 - 7 days | 2 - 3 days |
Notes
1. Contact us for other applications (i.e., single-cell, ppmSeq, ultra-deep metagenomic and metatranscriptomic sequencing, etc).
2. Conversion of non-Ultima libraries for UG 100 compatibility.
Tiers: Turnaround time (TAT) is in business days. TAT is for sequencing only and does not factor in the TAT for library prep or other companion services. Availability for the Priority Tier is limited; contact [email protected] to see if this is a current option. More information about Pricing Tiers.
Contact us with your for project specifications. Once project details are finalized, complete the appropriate submission form for submitting samples or submitting client-made libraries and email it to [email protected].
Campus drop-off locations:
- 1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
- 20 Snyder Hall (St. Paul campus)
Shipping information:
Samples should be frozen and shipped on dry ice in a 96-well plate. We recommend using plate tape to seal the wells. Place the plate inside of a plastic bag prior to placing on dry ice. Please give advance notice of submission date and time so staff can be prepared to receive samples. If shipping samples from outside the University of Minnesota, ship via express shipping carrier on dry ice to the address below.
UMN Genomics Center
ATTN: NGS Staff
3510 Hopkins Place N.
Building 4 Suite W402
Oakdale, MN 55128
612-625-7736
Please send the tracking information to [email protected].