DNA Library Preparation


Overview

DNA-Seq methods differ in terms of the DNA fragmentation method, genome size of interest, use of PCR amplification in library creation, and the use of “mate-pair” technology. All of the protocols listed in this section utilize Illumina reagents and protocols.

TruSeq Nano


This is a “standard” NGS workflow: 1) acoustic shearing of DNA, 2) enzymatic repair/ligation of adaptors, and 3) PCR amplification.

Nextera XT


This “transposase” method does not involve physical fragmentation of gDNA, but instead uses direct incorporation of Illumina adaptors via “tagmentation”.

This workflow is tailored to small genomes, such as those of bacteria. In both Nextera and Nextera XT methods, PCR is used to amplify successful tagmented molecules.

PCR-Free


This method is designed to avoid GC-bias associated with methods such as TruSeq and Nextera.

Questions?


Contact Elyse Froehling: [email protected].

Pricing

Expand all

UMN Rates

Illumina kit optionsSavings TierSpeed TierPriority Tier
Nextera XT. Quarter RXN. W/ library QC.$44.19$63.98$114.02
Turnaround time.10 - 15 days6 - 10 days3 - 5 days
Nextera XT. Quarter RXN. No library QC.  $35.20$50.02$78.26
Turnaround time.14 - 22 days8 - 13 days4 - 7 days
Nextera XT. Full-scale reaction.$84.47$115.37$184.70
Turnaround time.10 - 15 days6 - 10 days3 - 5 days
DNA Prep.$87.99$125.61$183.98
Turnaround time.10 - 15 days6 - 10 days3 - 5 days
TruSeq Nano.$107.20$195.63$234.48
Turnaround time.13 - 18 days7 - 13 days5 - 7 days
Other library prep optionsSavings TierSpeed TierPriority Tier
Tn-Seq $130.76$255.93$308.42
Turnaround time.13 - 18 days7 - 13 days5 - 7 days
ATAC-Seq$41.83$61.92$113.26
Turnaround time.10 - 15 days6 - 10 days3 - 5 days
Reagent. Transposase for ATAC-Seq.          $109.24N/AN/A

Notes
Tiers: Turnaround time (TAT) is in business days. TAT is for library prep only and does not factor in the TAT for sequencing or other companion services. Availability for the Priority Tier is limited; contact [email protected] to see if this is a current option. More information about Pricing Tiers.

External Rates

Illumina kit optionsSavings TierSpeed TierPriority Tier
Nextera XT. Quarter RXN. W/ library QC.$54.64$76.62$132.23
Turnaround time.10 - 15 days6 - 10 days3 - 5 days
Nextera XT. Quarter RXN. No library QC.  $43.52$59.98$91.37
Turnaround time.14 - 22 days8 - 13 days4 - 7 days
Nextera XT. Full-scale reaction.$104.45$138.78$215.79
Turnaround time.10 - 15 days6 - 10 days3 - 5 days
DNA Prep.$108.82$150.60$215.52
Turnaround time.10 - 15 days6 - 10 days3 - 5 days
TruSeq Nano.$132.56$230.79$274.03
Turnaround time.13 - 18 days7 - 13 days5 - 7 days
Other library prep optionsSavings TierSpeed TierPriority Tier
Tn-Seq $161.66$300.72$359.12
Turnaround time.13 - 18 days7 - 13 days5 - 7 days
ATAC-Seq$51.73$74.04$131.09
Turnaround time.10 - 15 days6 - 10 days3 - 5 days
Reagent. Transposase for ATAC-Seq.          $135.08N/AN/A

Notes
Tiers: Turnaround time (TAT) is in business days. TAT is for library prep only and does not factor in the TAT for sequencing or other companion services. Availability for the Priority Tier is limited; contact [email protected] to see if this is a current option. More information about Pricing Tiers.

Guidelines

Expand all

Submission

How to Order


1. Please contact [email protected] for project specifications.

2. Once project details are finalized, complete the appropriate submission form for submitting samples or submitting client-made libraries and email to [email protected].

NGS – Sample Submission Form

NGS – Pre-made Library Submission Form

Shipping Instructions


Samples should be frozen and shipped on dry ice in a 96-well plate. We recommend using plate tape to seal the wells. Place the plate inside of a plastic bag prior to placing on dry ice. Please give advance notice of submission date and time so staff can be prepared to receive samples. If shipping samples from outside the University of Minnesota, ship via express shipping carrier on dry ice to the address below.

Samples can be dropped off at one of our campus locations

Samples can be shipped to the following address:

Please send the tracking information to [email protected].

UMN Genomics Center
ATTN: NGS Staff
3510 Hopkins Place N.
Building 4 Suite W402
Oakdale, MN 55128
612-625-7736

FAQ

What is the turnaround time?


Generally, turnaround is 6-8 weeks for standard NGS projects. However, please contact [email protected] for specific project turnaround time.