Illumina DNA Library Preparation


Overview

DNA-Seq methods differ in terms of the DNA fragmentation method, genome size of interest, use of PCR amplification in library creation, and the use of “mate-pair” technology. All of the protocols listed in this section utilize Illumina reagents and protocols.

TruSeq Nano


This is a “standard” NGS workflow: 1) acoustic shearing of DNA, 2) enzymatic repair/ligation of adaptors, and 3) PCR amplification.

Nextera XT


This “transposase” method does not involve physical fragmentation of gDNA, but instead uses direct incorporation of Illumina adaptors via “tagmentation”.

This workflow is tailored to small genomes, such as those of bacteria. In both Nextera and Nextera XT methods, PCR is used to amplify successful tagmented molecules.

PCR-Free


This method is designed to avoid GC-bias associated with methods such as TruSeq and Nextera.

Questions?


Contact Elyse Froehling: next-gen@umn.edu.

Pricing

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Standard

Service Scale Unit UMN External
Illumina. Nextera XT. Quarter reaction.        
Standard Scale. With sample QC. 1-96 sample $38.11 $44.16
High Scale. With Sample QC. 97-any sample $34.60 $40.12
Standard Scale. No sample QC. 1-96 sample $26.73 $30.72
Standard Scale. No sample QC. 97-any sample $22.41 $27.62
Illumina Nextera XT. Full-scale reaction.        
Standard Scale. 1-96 sample $80.38 $92.81
High Scale. 97-any sample $69.47 $80.25
Illuimina Nextera DNA Flex.        
Standard Scale. 1-96 sample $84.55 $97.54
High Scale 97-any sample $75.61 $87.26
Illumina TruSeq Nano. 1-any sample $107.09 $123.40
Illumina PCR Free. 1-any sample $152.46 $175.55
Rubicon ThruPlex. 1-any sample $111.75 $128.89
Guidelines

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Submission

How to Order


Please contact next-gen@umn.edu for project specifications.

Once project details are finalized, complete appropriate submission form for submitting samples or submitting client-made libraries and email to next-gen@umn.edu.

Forms

Illumina Sequencing Request Form (Libraries)

Illumina Sequencing Request Form (Samples)

Samples can be dropped off at one of our laboratory locations.

1-202 Nils Hasselmo Hall (Minneapolis campus)

1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)

20 Snyder Hall (St. Paul campus)

Shipping Instruction

Samples should be frozen and shipped on dry ice in a 96-well plate. We recommend using plate tape to seal the wells. Place the plate inside of a plastic bag prior to placing on dry ice. Please give advance notice of submission date and time so staff can be prepared to receive samples. If shipping samples from outside the University of Minnesota, ship via express shipping carrier on dry ice to the address below.

If shipping samples, the following address should be used.

Please send the tracking information to next-gen@umn.edu.

University of Minnesota Genomics Center
1475 Gortner Avenue
28 Snyder Hall
St. Paul, MN 55108
612-625-7736

FAQ

What is the turnaround time?


Generally, turnaround is 6-8 weeks for standard NGS projects. However, please contact next-gen@umn.edu for specific project turnaround time.