Illumina DNA Library Preparation
DNA-Seq methods differ in terms of the DNA fragmentation method, genome size of interest, use of PCR amplification in library creation, and the use of “mate-pair” technology. All of the protocols listed in this section utilize Illumina reagents and protocols.
This is a “standard” NGS workflow: 1) acoustic shearing of DNA, 2) enzymatic repair/ligation of adaptors, and 3) PCR amplification.
This “transposase” method does not involve physical fragmentation of gDNA, but instead uses direct incorporation of Illumina adaptors via “tagmentation”.
This workflow is tailored to small genomes, such as those of bacteria. In both Nextera and Nextera XT methods, PCR is used to amplify successful tagmented molecules.
This method is designed to avoid GC-bias associated with methods such as TruSeq and Nextera.
Contact Elyse Froehling: firstname.lastname@example.org.
|Illumina. Nextera XT. Quarter reaction.|
|Standard Scale. With sample QC.||1-96||sample||$38.11||$44.16|
|High Scale. With Sample QC.||97-any||sample||$34.60||$40.12|
|Standard Scale. No sample QC.||1-96||sample||$26.73||$30.72|
|Standard Scale. No sample QC.||97-any||sample||$22.41||$27.62|
|Illumina Nextera XT. Full-scale reaction.|
|Illuimina Nextera DNA Flex.|
|Illumina TruSeq Nano.||1-any||sample||$107.09||$123.40|
|Illumina PCR Free.||1-any||sample||$152.46||$175.55|
How to Order
Please contact email@example.com for project specifications.
Once project details are finalized, complete appropriate submission form for submitting samples or submitting client-made libraries and email to firstname.lastname@example.org.
Samples can be dropped off at one of our laboratory locations.
1-202 Nils Hasselmo Hall (Minneapolis campus)
1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
20 Snyder Hall (St. Paul campus)
Samples should be frozen and shipped on dry ice in a 96-well plate. We recommend using plate tape to seal the wells. Place the plate inside of a plastic bag prior to placing on dry ice. Please give advance notice of submission date and time so staff can be prepared to receive samples. If shipping samples from outside the University of Minnesota, ship via express shipping carrier on dry ice to the address below.
If shipping samples, the following address should be used.
Please send the tracking information to email@example.com.
University of Minnesota Genomics Center
1475 Gortner Avenue
28 Snyder Hall
St. Paul, MN 55108