Genotyping-by-sequencing

The Genomics Center offers two robust GBS methods that differ slightly in their workflow. One, the in-line method, is based on the Poland protocol which has the barcodes in the adaptors to allow for sample multiplexing after ligation. We offer 376-plex with the in-line method using the PstI + MspI double digest.

The alternative method - and our most routinely used - has common adaptors that contain priming sites for Illumina multiplexing indices, so samples are barcoded during PCR amplification, and then pooled. The main advantages to this method are that it allows for highly multiplexed reactions and also enables us to provide greater restriction enzyme options to our clients.

Species We Have Worked With

We have worked extensively with many of the popular GBS plant species using industry-standard restriction enzymes, and we can use your previous GBS protocol if you need to merge new data with a previous data set. In addition, we have optimized new GBS protocols for over 100 non-model species, giving researchers a new, low-cost genotyping option for their species. Please see our pilot project page for more information on optimizing a GBS protocol for your species.

Optional Add-on Services

The Genomics Center offers an end-to-end GBS service starting from DNA extraction through variant calling. Our highly experienced extraction team provides unparalleled high throughput extraction services and are able to customize protocols when needed. Please see our extraction page for pricing and contact information.

In addition, we offer a standard variant calling informatic service for diploid species using the STACKS pipeline. Deliverables include a GBS report as well as raw demultiplexed fastq files and filtered genotype calls in VCF format. If you are interested in further analyses beyond what is included in our standard analysis report, please discuss your project goals with your GBS project manager prior to starting your project. All additional analyses are billed at an hourly rate. 

Start a Project

Whether your species is new to GBS or has an established protocol, we can help determine which sequencing instrument will best meet your budget and project goals. Clients are encouraged to contact our experienced GBS team for a consult on experimental set-up and sequencing options at [email protected]. Please tell us about your project by filling out our GBS project inquiry form to start your project today.

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