High-throughput Q-RT-PCR on the BioMark HD


Overview

The Fluidigm BioMark HD is a microfluidic, high-throughput instrument for real-time Q-RT-PCR of up to 9,216 reactions in a single run. The heart of the platform is a microfluidic chip called an “Integrated Fluidic Circuit (IFC)” into which are deposited amplification-ready templates (e.g., cDNA) and gene-specific assays (e.g., Taqman). The IFC performs automatic mixing of templates and assays in nanoliter amplification wells, followed by real-time PCR and data capture on the BioMark HD.

High-throughput IFCs can be run at the UMGC in three formats (# templates vs # assays): 1) 96.96 (9,216 reactions), 2) 48.48 (2,304 reactions) and 3) 192.24 (4,608 reactions). A fourth IFC also exists – the “FLEXsix IFC” – containing six independent 12.12 arrays that can be run independently for small-scale experiments or pilot runs.

Use of the Fluidigm BioMark involves two costs: 1) the IFC itself, 2) Chip processing. Chip processing may be performed by the UMGC (high-throughput IFCs), or by clients themselves, who therefore pay only for the instrument access (FLEXsix IFC).

Questions?


Contact Darrell Johnson: exprn@umn.edu.

Pricing

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Standard

Service Scale Unit UMN External
Fluidigm BioMark HD Chip Processing.
By client. 1-any run $304.36 $458.89
By UMGC. 1-any run $681.56 $1,027.59
Fluidigm BioMark IFCs.
FLEXsix Chip and Loading Reagents.1 1-any each $631.60 $952.28
48.48 (samples.assays). 1-any each $655.04 $987.62
96.96 (samples.assays). 1-any each $2,101.18 $3,167.99
192.24 (samples.assays). 1-any each $1,061.82 $1,600.92
Fluidigm DELTAgene Assays.
Real-time Q-PCR assay. 12-any gene $64.28 $96.91
Wet-lab tested.2 12-any gene $160.69 $242.27
Notes
  1. The FLEXsix IFC is designed for smaller-scale, pilot, and optimization experiments, and contains six independent 12.12 (samples.assays) arrays of reactions chambers that can be run simultaneously, or in separate runs.
  2. Each gene-specific DELTAgene assays provide adequate material for up to 100 IFCs. Wet-lab tested assay have been functionally validated by Fluidigm.
  3. PRICING FOR EXTERNAL CLIENTS WILL VARY FROM PUBLISHED RATE. Please contact the UMGC to obtain a quote.
Guidelines

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Submission

How to Order


Contact exprn@umn.edu for project specifications.

If running your own IFC on the BioMark, please fill out the submission form prior to scheduling a run.

Deliverables

Deliverables


Client is emailed a data package.