High-throughput Q-RT-PCR on the BioMark HD
The Fluidigm BioMark HD is a microfluidic, high-throughput instrument for real-time Q-RT-PCR of up to 9,216 reactions in a single run. The heart of the platform is a microfluidic chip called an “Integrated Fluidic Circuit (IFC)” into which are deposited amplification-ready templates (e.g., cDNA) and gene-specific assays (e.g., Taqman). The IFC performs automatic mixing of templates and assays in nanoliter amplification wells, followed by real-time PCR and data capture on the BioMark HD.
High-throughput IFCs can be run at the UMGC in three formats (# templates vs # assays): 1) 96.96 (9,216 reactions), 2) 48.48 (2,304 reactions) and 3) 192.24 (4,608 reactions). A fourth IFC also exists – the “FLEXsix IFC” – containing six independent 12.12 arrays that can be run independently for small-scale experiments or pilot runs.
Use of the Fluidigm BioMark involves two costs: 1) the IFC itself, 2) Chip processing. Chip processing may be performed by the UMGC (high-throughput IFCs), or by clients themselves, who therefore pay only for the instrument access (FLEXsix IFC).
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|Fluidigm BioMark HD Chip Processing.|
|Fluidigm BioMark IFCs.|
|FLEXsix Chip and Loading Reagents.1||1-any||each||$631.60||$952.28|
|Fluidigm DELTAgene Assays.|
|Real-time Q-PCR assay.||12-any||gene||$64.28||$96.91|
- The FLEXsix IFC is designed for smaller-scale, pilot, and optimization experiments, and contains six independent 12.12 (samples.assays) arrays of reactions chambers that can be run simultaneously, or in separate runs.
- Each gene-specific DELTAgene assays provide adequate material for up to 100 IFCs. Wet-lab tested assay have been functionally validated by Fluidigm.
- PRICING FOR EXTERNAL CLIENTS WILL VARY FROM PUBLISHED RATE. Please contact the UMGC to obtain a quote.