BioMark Q-RT-PCR
The Fluidigm BioMark HD is a microfluidic, high-throughput instrument for real-time Q-RT-PCR of up to 9,216 reactions in a single run. The heart of the platform is a microfluidic chip called an “Integrated Fluidic Circuit (IFC)” into which are deposited amplification-ready templates (e.g., cDNA) and gene-specific assays (e.g., Taqman). The IFC performs automatic mixing of templates and assays in nanoliter amplification wells, followed by real-time PCR and data capture on the BioMark HD.
High-throughput IFCs can be run at the UMGC in three formats (# templates vs # assays): 1) 96.96 (9,216 reactions), 2) 48.48 (2,304 reactions) and 3) 192.24 (4,608 reactions). A fourth IFC also exists – the “FLEXsix IFC” – containing six independent 12.12 arrays that can be run independently for small-scale experiments or pilot runs.
Use of the Fluidigm BioMark involves two costs: 1) the IFC itself, 2) Chip processing. Chip processing may be performed by the UMGC (high-throughput IFCs), or by clients themselves, who therefore pay only for the instrument access (FLEXsix IFC).
Questions?
Contact us at exprn@umn.edu.
Standard
| Service | Scale | Unit | UMN | External |
|---|---|---|---|---|
| Fluidigm BioMark HD Chip Processing. | ||||
| By client. | 1-any | run | $304.36 | $458.89 |
| By UMGC. | 1-any | run | $681.56 | $1,027.59 |
| Fluidigm BioMark IFCs. | ||||
| FLEXsix Chip and Loading Reagents.1 | 1-any | each | $631.60 | $952.28 |
| 48.48 (samples.assays). | 1-any | each | $655.04 | $987.62 |
| 96.96 (samples.assays). | 1-any | each | $2,101.18 | $3,167.99 |
| 192.24 (samples.assays). | 1-any | each | $1,061.82 | $1,600.92 |
| Fluidigm DELTAgene Assays. | ||||
| Real-time Q-PCR assay. | 12-any | gene | $64.28 | $96.91 |
| Wet-lab tested.2 | 12-any | gene | $160.69 | $242.27 |
- The FLEXsix IFC is designed for smaller-scale, pilot, and optimization experiments, and contains six independent 12.12 (samples.assays) arrays of reactions chambers that can be run simultaneously, or in separate runs.
- Each gene-specific DELTAgene assays provide adequate material for up to 100 IFCs. Wet-lab tested assay have been functionally validated by Fluidigm.
- PRICING FOR EXTERNAL CLIENTS WILL VARY FROM PUBLISHED RATE. Please contact the UMGC to obtain a quote.
Submission
How to Order
Contact exprn@umn.edu for project specifications.
If running your own IFC on the BioMark, please fill out the submission form prior to scheduling a run.
Deliverables
Deliverables
Client is emailed a data package.