DNA Extraction
The UMGC offers DNA extraction using a wide range of commercially available kits, bead-beating methods, and homebrew protocols developed at the UMGC for high-throughput extraction in 96-well plate format.
We routinely extract DNA from the following tissue sources. If you have tissue not listed below, please contact us.
- Whole Blood – The UMGC processes bulk whole blood samples for gDNA using a “salting out” method that has been in place at the UMN’s hospital clinic for two decades, and delivers very high yields.
- Saliva – Oragene Collection kits
- Buccal swabs
- FTA filter paper
- Plant and Animal Tissue
- Environmental and Fecal
- Cell Pellets
- Bacteria
The assessment of DNA purity and concentration by UV spectroscopy (NanoDrop) and fluorimetry (PicoGreen quantification) is included in the price of most services.
Questions?
Contact: extract@umn.edu.
Standard
| Service | Scale | Unit | UMN | External |
|---|---|---|---|---|
| Microbial. Qiagen DNeasy PowerSoil Pro. | ||||
| Individual. | 1-any | sample | $34.65 | $40.13 |
| Standard scale. | 49-96 | sample | $23.78 | $27.61 |
| High scale. Plate of 96. | 1-any | plate | $2,138.88 | $2,485.44 |
| Plant. Qiagen DNeasy Plant HT. | ||||
| Low scale. | 1-any | sample | $15.97 | $18.63 |
| Standard scale. Plate of 96. | 1-any | plate | $1,322.88 | $1,546.56 |
| Plant. CTAB Method. | ||||
| Individual. | 1-any | sample | $13.05 | $15.28 |
| Standard scale. Plate of 96. | 1-any | plate | $1,285.63 | $1,504.27 |
| Tissue/Blood/Body Fluids. Qiagen DNeasy or QIAamp. | ||||
| Individual. | 1-any | sample | $23.60 | $27.41 |
| HTP. | 1-any | sample | $12.60 | $14.76 |
| GBS. Standard Extractions.1 | 1-any | sample | $11.16 | $12.83 |
Notes
- GBS extractions include quantification by Nanodrop only. All other DNA extraction services include both Nanodrop and Picogreen quantification.
Submission
How to Order
- Before submitting samples for extraction, please contact extract@umn.edu for project specifications.
- Guidelines on sample submission can be found here.
- Complete the sample submission form and email to extract@umn.edu.
Samples can be dropped off at any of our campus locations
- 1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
- 20 Snyder Hall (St. Paul campus)
Shipping information
Please send the tracking information to extract@umn.edu.
UMN Genomics Center
ATTN: Jerry Daniel/Veronica Tonnell
3510 Hopkins Place N.
Building 4 Suite W402
Oakdale, MN 55128
651-374-1227 (ext. 5)
Deliverables
Client receives a QC report with sample details.
FAQ
What is the turnaround time?
Please contact extract@umn.edu for a specific project turnaround time.
What is the turnaround time?
It depends upon sample numbers. We handle both small (1-2 samples) and large (in the thousands). Please contact us for a timeline.
What is your recommendation on shipping?
- Dry Ice – Cells, Tissue, Blood, Fecal and Plant
- Room Temp – Buccal Dried Swabs, Oragene kits, and FTA cards
What is your shipping address?
UMN Genomics Center
ATTN: Jerry Daniel/Veronica Tonnell
3510 Hopkins Place N.
Building 4 Suite E445A
Oakdale, MN 55128
651-374-1227 (ext. 5)
How do you determine the quality of extracted DNA?
The assessment of DNA purity and concentration is by both UV spectroscopy (NanoDrop) and fluorimetry (PicoGreen quantification), usually included in most of DNA extraction services. Upon request, we can also provide DNA sizing either via Gel or Agilent Bio-analyzer.
What type extractions do you offer?
We use a wide range of commercially available kits, bead beating methods as well as Home-Brew methods, listed below are some common methods. If you have your own method please contact us.
- QIAGEN DNeasy: a standard, lower-cost silica-binding purification method that provides DNA of excellent quality for genomic applications.
- ORAGENE BUCCAL: Oragene buccal cell collection kits offer pain-free and noninvasive collection that is readily self-administered, suitable for shipment in standard mail, and stable at room temperature.
- QUICK-AND-EASY: low-cost extraction suitable for downstream mouse genotyping. The output is a semi-purified DNA prep that compatible with PCR-based assays. The method forgoes quantification and purification in order to achieve the lowest cost possible.
- QIAGEN DNeasy POWERSOIL (formerly MOBIO POWERSOIL): a “bead-beating” method that uses abrasive beads to break the cell walls of microbes in stool and other samples. This service is typically used in combination with 16S/18S sequencing.
- HIGH-THROUGHPUT PLANT: a homebrew method developed at the UMGC for high-throughput extraction of DNA from plant material submitted in 96-well plate format. Please inquire for details.
- QIAGEN MagAttract PowerMicrobiome DNA/RNA: a “bead-beating” method that uses abrasive beads to break the cell walls of microbes in stool and other samples. Extracts both RNA and DNA This service is typically used in combination with 16S/18S/Std NGS Seq sequencing.
Can you Store our samples?
UMGC has the capability of archiving your sample with 2D-Barcoded tubes/Plates and a retrieval system. This also enables us to re-rack/cherry-pick samples for future downstream applications at the UMGC or ship them to an off-site location.