The NanoString nCounter is a single-molecule counting device for the digital quantification of up to 800 different genes in a single multiplexed reaction. The technology is based on gene-specific probe pairs that are hybridized to the sample in combination with automated imaging and detection. The protocol eliminates any enzymatic reactions that might introduce bias in the results.
nCounter assays can be used with a wide variety of different sample types such as purified total RNA, raw cell or blood lysates, and FFPE tissue sections with no loss in precision. Severely degraded RNA can be used, owing to the fact that the nCounter probe sets only hybridize to a 100 base pair contiguous region of the target oligonucleotide.
Key benefits
- Highly multiplexed - analyze the expression of up to 800 RNA, DNA, or protein targets.
- Sample flexibility - works with purified total RNA, raw cell or blood lysates, and FFPE tissue sections with no loss in precision.
- Zero enzymatic steps - direct, digital quantification with no amplification or cDNA conversion.
- Simple data analysis - nCounter data can be quickly analyzed using openly available nSolver and ROSALIND tools.
Questions?
To start a project or to learn more custom CodeSet options, contact us at [email protected].
There are 30 off-the-shelf panels available for gene expression, as well as additional off-the shelf panels for gene fusions, miRNA, CNV, and protein detection. Or design your own multiplex Custom CodeSet with up to 800 user-defined targets for a variety of organisms and applications. Commonly requested panels:
| Panels | Species | Genes | Application |
|---|---|---|---|
| Inflammation | Human, Mouse | 255, 254 | Immunology |
| Immunology | Human, Mouse | 594, 561 | Immunology |
| Glial Profiling | Human, Mouse | 770 | Neurology |
| miRNA | Human, Mouse, Rat | 827, 577, 423 | Immunology |
| Canine IO | Canine | 800 | Oncology |
| Custom | Any | 800 | Any |
See NanoString's Panel Pro Selection Tool for the full catalog of inventoried and made-to-order gene expression panels by name, application area, biologic pathway/process, or gene name(s).
UMN Rates
| Service | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| nCounter Expression Analysis. Processing. | $15.22 | $20.64 | $44.53 |
| Turnaround time. | 14 - 24 days | 8 - 13 days | 4 - 6 days |
| Sample volume. | 12 - 96 samples | 12 - 36 samples | 12 - 24 samples |
| nCounter miRNA. Processing. | $21.47 | $31.04 | $62.84 |
| Turnaround time. | 24 - 34 days | 13 - 23 days | 6 - 11 days |
| Sample volume. | 12 - 96 samples | 12 - 48 samples | 12 - 24 samples |
Notes
Price: Excludes CodeSet cost, but includes labor, instrument time, and “universal” reagents.
Tiers: Turnaround time (TAT) is in business days. TAT is for library prep only and does not factor in the TAT for companion services. Availability for the Priority Tier is limited; contact [email protected] to see if this is a current option. More information about Pricing Tiers.
External Rates
| Service | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| nCounter Expression Analysis. Processing. | $19.46 | $25.85 | $52.38 |
| Turnaround time. | 14 - 24 days | 8 - 13 days | 4 - 6 days |
| Sample volume. | 12 - 96 samples | 12 - 36 samples | 12 - 24 samples |
| nCounter miRNA. Processing. | $28.05 | $38.67 | $74.00 |
| Turnaround time. | 24 - 34 days | 13 - 23 days | 6 - 11 days |
| Sample volume. | 12 - 96 samples | 12 - 48 samples | 12 - 24 samples |
Notes
Price: Excludes CodeSet cost, but includes labor, instrument time, and “universal” reagents.
Tiers: Turnaround time (TAT) is in business days. TAT is for library prep only and does not factor in the TAT for companion services. Availability for the Priority Tier is limited; contact [email protected] to see if this is a current option. More information about Pricing Tiers.
Submission
How to Order
Contact [email protected] for project specifications.
Project submission guidelines and submission form can be found here. Please email the completed form to [email protected] and print the “NanoString Project Information” section under tab 3 and submit with your samples.
Samples can be dropped off at one of our laboratory locations.
1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
20 Snyder Hall (St. Paul campus)
If shipping samples, the following address should be used.
Please send the tracking information to [email protected].
UMN Genomics Center
ATTN: Darrell Johnson
3510 Hopkins Place N.
Building 4 Suite W402
Oakdale, MN 55128
612-625-7736
Deliverables
Client is emailed a data package from nSolver analysis software. This data package will contain:
- *.csv files for non-normalized data.
- *.csv files for normalized data.
- An *.html file containing the experiment report.
- *.RCC Files. These are instrument files for performing your own custom analysis.
FAQs
Is software available for nCounter data?
Yes, nSolver can be downloaded for free at http://www.nanostring.com/products/nSolver