NGS Library Manipulation


Overview

It may be desirable for your lab to make libraries outside of the UMGC, either to reduce costs or to implement protocols that are not offered as standard services by the UMGC. In order to help researchers with their own library creation efforts, the UMGC offers a variety of DNA manipulation services “à la carte”. These include DNA shearing, LabChip XT size-selection, and library pooling and quality control.

Questions?


Contact Elyse Froehling: next-gen@umn.edu.

Pricing

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UMN Rates

DNA ShearingSavings TierSpeed TierPriority Tier
Covaris Long (>20 min) Shear. w/ Sizing.$64.88$112.08$159.91
Turnaround time.5 to 8 days3 to 6 days2 to 3 days
Covaris. w/ Sizing.$36.71$55.07$85.15
Turnaround time.5 to 8 days3 to 6 days2 to 3 days
DNA Clean-upSavings TierSpeed TierPriority Tier
Ampure bead. No QC.                                 $10.75$17.20$20.43
Turnaround time.3 to 6 days2 to 4 days1 to 2 days


Notes
Tiers: Turnaround time (TAT) is in business days. Availability for the Priority Tier is limited; contact next-gen@umn.edu to see if this is a current option.

External Rates

DNA ShearingSavings TierSpeed TierPriority Tier
Covaris Long (>20 min) Shear. w/ Sizing.$82.77$135.26$188.43
Turnaround time.5 to 8 days3 to 6 days2 to 3 days
Covaris. w/ Sizing.$46.82$67.23$100.64
Turnaround time.5 to 8 days3 to 6 days2 to 3 days
DNA Clean-upSavings TierSpeed TierPriority Tier
Ampure bead. No QC.                                 $13.73$20.88$24.48
Turnaround time.3 to 6 days2 to 4 days1 to 2 days


Notes
Tiers: Turnaround time (TAT) is in business days. Availability for the Priority Tier is limited; contact next-gen@umn.edu to see if this is a current option.

Guidelines

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Submission

How to Order


Covaris DNA Shearing

Please print and submit a completed form with your samples:
Covaris Shearing Internal Submission Form
Covaris Shearing External Submission Form

Caliper LabChip XT Size Selection or Library Pooling

Please contact next-gen@umn.edu for project specifications.

Shipping Instructions


Samples should be frozen and shipped on dry ice in a 96-well plate. We recommend using plate tape to seal the wells. Place the plate inside of a plastic bag prior to placing on dry ice. Please give advance notice of submission date and time so staff can be prepared to receive samples. If shipping samples from outside the University of Minnesota, ship via express shipping carrier on dry ice to the address below.

Samples can be dropped off at one of our campus locations

1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)

20 Snyder Hall (St. Paul campus)

Samples can be shipped to the following address:

Please send the tracking information to next-gen@umn.edu.

UMN Genomics Center
ATTN: Nasia Mead
3510 Hopkins Place N.
Building 4 Suite W402
Oakdale, MN 55128
612-625-7736

FAQ

What is the turnaround time?


Please contact next-gen@umn.edu for specific project turnaround time.