NGS Library Manipulation
It may be desirable for your lab to make libraries outside of the UMGC, either to reduce costs or to implement protocols that are not offered as standard services by the UMGC. In order to help researchers with their own library creation efforts, the UMGC offers a variety of DNA manipulation services “à la carte”. These include DNA shearing, LabChip XT size-selection, and library pooling and quality control.
Questions?
Contact Elyse Froehling: next-gen@umn.edu.
Standard
| Service | Scale | Unit | UMN | External |
|---|---|---|---|---|
| DNA Shearing. Covaris. | ||||
| This rate includes Agilent DNA sizing. | 8-any | sample | inquire | inquire |
| Caliper LabChip XT Size Selection. DNA. | ||||
| This rate includes Agilent DNA sizing. | 1-any | sample | inquire | inquire |
| Library Pooling. 96-well plate. | 1-any | plate of 96 | inquire | inquire |
Submission
How to Order
Covaris DNA Shearing
Please print and submit a completed form with your samples:
Covaris Shearing Internal Submission Form
Covaris Shearing External Submission Form
Caliper LabChip XT Size Selection or Library Pooling
Please contact next-gen@umn.edu for project specifications.
Shipping Instructions
Samples should be frozen and shipped on dry ice in a 96-well plate. We recommend using plate tape to seal the wells. Place the plate inside of a plastic bag prior to placing on dry ice. Please give advance notice of submission date and time so staff can be prepared to receive samples. If shipping samples from outside the University of Minnesota, ship via express shipping carrier on dry ice to the address below.
Samples can be dropped off at one of our campus locations
1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
20 Snyder Hall (St. Paul campus)
Samples can be shipped to the following address:
Please send the tracking information to next-gen@umn.edu.
University of Minnesota Genomics Center
1475 Gortner Ave.
28 Snyder Hall
St. Paul, MN 55108
612-625-7736