NGS Library Manipulation
It may be desirable for your lab to make libraries outside of the UMGC, either to reduce costs or to implement protocols that are not offered as standard services by the UMGC. In order to help researchers with their own library creation efforts, the UMGC offers a variety of DNA manipulation services “à la carte”. These include DNA shearing, LabChip XT size-selection, and library pooling and quality control.
Questions?
Contact Elyse Froehling: next-gen@umn.edu.
Standard
| Service | Scale | Unit | UMN | External |
|---|---|---|---|---|
| DNA Shearing. Covaris. | ||||
| This rate includes Agilent DNA sizing. | 8-any | sample | $31.66 | $36.63 |
| Caliper LabChip XT Size Selection. DNA. | ||||
| This rate includes Agilent DNA sizing. | 1-any | sample | $79.62 | $91.68 |
| Library Pooling. 96-well plate. | 1-2 | plate of 96 | $93.00 | $107.69 |
| 3-4 | plate of 96 | $68.94 | $81.47 | |
| 5-any | plate of 96 | $47.88 | $58.52 |
Submission
How to Order
DNA Shearing. Covaris.
Please print and submit completed form with your samples.
Researchers outside the University can request a form by emailing next-gen@umn.edu.
Forms
Print and submit completed form with your samples.
Covaris Shearing Internal Submission Form
Covaris Shearing External Submission Form
Caliper LabChip XT Size Selection or Library Pooling
Please contact next-gen@umn.edu for project specifications.
Once project details are finalized, complete the appropriate submission form for submitting samples or submitting client-made libraries and email to next-gen@umn.edu.
Samples can be dropped off at one of our laboratory locations.
1-202 Nils Hasselmo Hall (Minneapolis campus)
1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
20 Snyder Hall (St. Paul campus)
Shipping Instructions
Samples should be frozen and shipped on dry ice in a 96-well plate. We recommend using plate tape to seal the wells. Place the plate inside of a plastic bag prior to placing on dry ice. Please give advance notice of submission date and time so staff can be prepared to receive samples. If shipping samples from outside the University of Minnesota, ship via express shipping carrier on dry ice to the address below:
If shipping samples, the following address should be used.
Please send the tracking information to next-gen@umn.edu.
University of Minnesota Genomics Center
1475 Gortner Avenue
28 Snyder Hall
St. Paul, MN 55108
612-625-7736