The Illumina MiSeq is a benchtop sequencer specialized for quick, lower-output runs on single-lane flow cells, and with the version 3 cluster chemistry, it offers Illumina’s longest read length - the 300-bp paired-end run.
Optimized for projects requiring 1–16 million reads per run, the MiSeq offers a lower-throughput alternative to the NextSeq P1 flow cell, which generates 100 million reads per run. Its single-lane design enables fast sequencing turnaround times, particularly when paired with our Speed or Priority service tiers.
Questions?
Contact [email protected] with any questions about our low throughput sequencing options.
UMN Rates
| 150-cycle (75 PE) | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| MiSeq. v3. 16 - 22 M reads/lane. | $1,454.76 | $1,777.59 | $2,013.52 |
| Turnaround time. | 9 - 14 days | 7 - 11 days | 5 - 6 days |
| 300-cycle (150 PE) | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| MiSeq. Nano. 1 M reads/lane. | $616.15 | $784.59 | $956.14 |
| Turnaround time. | 8 - 13 days | 5 - 7 days | 4 - 5 days |
| MiSeq. Micro v2. 4 M reads/lane. | $834.87 | $1,043.58 | $1,231.92 |
| Turnaround time. | 8 - 13 days | 6 - 10 days | 4 - 5 days |
| MiSeq. v2. 8 - 12 M reads/lane. | $1,727.86 | $2,100.98 | $2,357.87 |
| Turnaround time. | 9 - 14 days | 7 - 11 days | 5 - 6 days |
| 500-cycle (250 PE) | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| MiSeq. v2. 8 - 12 M reads/lane. | $1,919.39 | $2,327.75 | $2,599.35 |
| Turnaround time. | 9 - 14 days | 7 - 11 days | 5 - 6 days |
| 600-cycle (300 PE) | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| MiSeq. v3. 16 - 22 M reads/lane. | $2,441.80 | $2,946.35 | $3,258.05 |
| Turnaround time. | 9 - 14 days | 7 - 11 days | 5 - 6 days |
Notes
1. Output: In an effort to improve data quality, the output capacity for custom libraries, including amplicon libraries and low-diversity applications, may generate up to 25% less data due to the addition of PhiX.
2. Tiers: Turnaround time (TAT) is in business days. TAT is for sequencing only and does not factor in the TAT for library prep or other companion services. Availability for the Priority Tier is limited; contact [email protected] to see if this is a current option. More information about Pricing Tiers.
External Rates
| 150-cycle (75 PE) | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| MiSeq. v3. 16 - 22 M reads/lane. | $1,798.90 | $2,157.61 | $2,419.00 |
| Turnaround time. | 9 - 14 days | 7 - 11 days | 5 - 6 days |
| 300-cycle (150 PE) | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| MiSeq. Nano. 1 M reads/lane. | $761.90 | $949.06 | $1,139.47 |
| Turnaround time. | 8 - 13 days | 5 - 7 days | 4 - 5 days |
| MiSeq. Micro v2. 4 M reads/lane. | $1,032.35 | $1,264.26 | $1,473.17 |
| Turnaround time. | 8 - 13 days | 6 - 10 days | 4 - 5 days |
| MiSeq. v2. 8 - 12 M reads/lane. | $2,136.61 | $2,551.19 | $2,835.69 |
| Turnaround time. | 9 - 14 days | 7 - 11 days | 5 - 6 days |
| 500-cycle (250 PE) | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| MiSeq. v2. 8 - 12 M reads/lane. | $2,373.45 | $2,827.19 | $3,127.90 |
| Turnaround time. | 9 - 14 days | 7 - 11 days | 5 - 6 days |
| 600-cycle (300 PE) | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| MiSeq. v3. 16 - 22 M reads/lane. | $3,019.45 | $3,580.08 | $3,925.00 |
| Turnaround time. | 9 - 14 days | 7 - 11 days | 5 - 6 days |
Notes
1. Output: In an effort to improve data quality, the output capacity for custom libraries, including amplicon libraries and low-diversity applications, may generate up to 25% less data due to the addition of PhiX.
2. Tiers: Turnaround time (TAT) is in business days. TAT is for sequencing only and does not factor in the TAT for library prep or other companion services. Availability for the Priority Tier is limited; contact [email protected] to see if this is a current option. More information about Pricing Tiers.
Submission
How to order
Download the appropriate submission form for submitting samples or pre-made libraries. Once the form has been completed, please submit the submission form by emailing [email protected].
Forms
NGS – Pre-made Library Submission Form
Samples can be dropped off at one of our campus locations
1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
20 Snyder Hall (St. Paul campus)
Samples can be shipped to the following address:
Please send the tracking information to [email protected].
UMN Genomics Center
ATTN: NGS Staff
3510 Hopkins Place N.
Building 4 Suite W402
Oakdale, MN 55128
612-625-7736
Deliverables
Data Release
There are four options for transferring data from the UMGC to clients: 1) delivery to the Minnesota Supercomputing Institute’s (MSI) high-performance file system, 2) download from a secure website, 3) download with Globus, or 4) shipment on an external hard drive. Please indicate your data delivery preference when placing an order for sequencing.
1. MSI storage
Internal clients have their data released to MSI's Shared User Resource Facility Storage (SURFS). Delivered data will be located in the "data_delivery" folder in your group's folder on MSI's primary filesystem (home/GROUP/data_delivery/umgc). MSI does not charge for SURFS storage costs, but files expire and are removed one year after they've been delivered. Files should be copied to other MSI storage locations such as Tier2, Tier3, or your group's "shared" folder before they expire.
2. Web download
Internal clients that opt-out of MSI storage and external clients can download their data from a secure website using either a web browser or a command-line download tool, complete instructions are provided in an email from the UMGC. The client’s data is available for download for 30 days, after which the data will be removed from the data download website and the client takes responsibility for storing the data.
3. Globus
Internal and External clients can use Globus to download their data. This is the recommended method for external clients to download large datasets.
4. Hard drive
External clients may have data shipped on a hard drive purchased by the UMGC and invoiced to the client at a cost of $250 per hard drive.
Data Recovery
The UMGC archives most customer data for a year and some datasets are retained for 5 years or more. If you need a dataset re-delivered email a request to [email protected] to initiate data recovery. The UMGC does not provide any guarantee that data can be successfully recovered from the archive.
FAQs
Do you accept client-made libraries?
Yes, please consult with UMGC staff before submitting client-made libraries by emailing [email protected].
We recommend that pooling be done at UMGC as PicoGreen concentrations are used for balancing libraries. However, clients may choose to pool themselves.
Client-made libraries will have the following services performed on them before proceeding with sequencing.
- PicoGreen (concentration of library)
- DNA Agilent High Sensitivity chip (size of library)
- KAPA QC (functionality of library)