Curio Seeker, built on Slide-seq technology, is a high-resolution spatial mapping solution for whole-transcriptome analysis in fresh frozen tissue. Without the need for traditional microscopy-based approaches, Curio Seeker enables researchers to generate detailed tissue images by molecularly profiling individual cells in their spatial location using next-generation sequencing. Commercialized by Curio Bioscience, with development at the Broad Institute and Harvard, Curio Seeker provides a user-friendly workflow for researchers to explore cell-environment interactions at single-cell resolution without requiring specialized instrumentation.

Explore the UMN Spatialomics Initiative website to learn more about the Seeker platform and to compare technical details to other spatial platforms.

Service Details

• Two library prep options: 1) Researchers can receive training on the Seeker workflow through the UMGC CoLab. Libraries can then be prepared either in the CoLab space or within the researcher’s own lab. 2) Researchers can submit tissue samples mounted on slides to UMGC Spatial Services, where library preparation and sequencing will be performed.
• Sequence compatibility: The UMGC sequences Seeker libraries on the Illumina NovaSeq X Plus or the low-cost Element AVITI instruments. The choice of sequencing platform depends on the project size: smaller projects are more cost-effective on the Aviti platform, while larger projects are better suited for the NovaSeq X Plus.
• Streamlined bioinformatics workflow: The Curio Seeker bioinformatics pipeline processes FASTQ files generated from Curio Seeker sequencing libraries into spatial gene expression maps in a single, efficient package. The UMGC will provide output files that are compatible with third-party analysis and visualization tools. 

Questions

Contact Dr. Fernanda Rodriguez at [email protected] with questions on the Curio Seeker platform or to schedule a consultation.