DNA methylation analysis is a powerful tool for exploring gene regulation, cellular behavior, and the molecular basis of disease. Illumina’s Infinium Methylation microarrays provide high-throughput, single-CpG resolution profiling across the genome, delivering precise and quantitative insights into methylation patterns. This technology is widely applied in cancer biology, aging research, neuroscience, studies of rare diseases, and environmental epigenetics.
The UMGC is one of the few service providers in the U.S. offering the latest Illumina Methylation Screening Array (MSA), optimized for epigenome-wide association studies (EWAS). This platform combines broad genomic coverage with exceptional reproducibility and cost efficiency. Using bisulfite conversion, the assay differentiates methylated from unmethylated cytosines, followed by site-specific probe detection and fluorescent labeling to quantify methylation with high accuracy.
Advantages of Methylation Microarrays
Methylation microarrays combine comprehensive coverage and high-throughput capabilities. Advantages include:
- Comprehensive genome-wide coverage that includes CpG islands, non-CpG and differentially methylated sites, enhancers, open chromatin, transcription factor binding sites, miRNA promoter regions
- Assay reproducibility
- Cost-effective and scalable
Illumina Methylation Microarrays
Methylation Screening Array
Illumina’s Methylation Screening Array is a 48-sample BeadChip designed for population-scale screening and offers the following benefits:
- Contains ~270K markers enriched for non-cancer diseases, environmental exposures, and population-level biological traits
- Overlap of ~145K markers with MethylationEPIC v2.0
- Lower cost (per-sample price ~50% of MethylationEPIC v2.0)
- High-quality data with >98% reproducibility
- User-friendly data analysis pipelines
- Compatible with public databases and powerful disease classifiers
MethylationEPIC v2.0
Illumina’s MethylationEPIC v2.0, successor to the gold standard Methylation 850K array, provides researchers the ability to interrogate the same high-impact CpG sites but includes some upgrades. MethylationEPICv2.0 offers:
- Coverage of ~930K unique sites located in enhancer regions, gene bodies, promoters, and CpG islands
- Over 186,000 new probes were designed to target known enhancers, super-enhancers, CTCF-binding domains, and open regions of chromatin associated with primary tumors
- Comprehensive, genome-wide coverage ideal for genetic disease testing
- Maintains high backward compatibility with MethylationEPIC v1.0 (850K)
- Compatible with DNA extracted from FFPE tissue samples
Mouse Methylation BeadChip
Illumina’s Mouse Methylation BeadChip is designed for compatibility across a broad range of applications, including epigenome-wide association studies, xenograft experiments, preclinical research, and more.
- Developed to support the laboratories using mouse model systems to mimic disease states in humans
- Interrogates 285K methylation sites per sample at single-nucleotide resolution
- Offers balanced coverage of CpG islands, transcription start sites (TSS), enhancers, imprinted loci, gene body regions, repetitive element regions, lamin attachment domains, CTCF binding sites, and hypermethylated regions in cancer
- Enables epigenetic analysis of virtually all murine strains used in research laboratories
- Provides a PCR-free protocol using the powerful Infinium HD Assay for epigenetic studies
Getting Started
With a commitment to quality and scalability, UMGC provides researchers with the high-resolution methylation data needed to investigate epigenetic changes. For microarray inquiries, please contact [email protected].
UMN Rates
| Service | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Infinium Human MethylationEPIC. | $233.67 | $276.23 | $290.14 |
| Turnaround time. | 26 to 60 days | 15 to 26 days | 8 to 12 days |
| Infinium Human Methylation Screening Array. | $117.47 | $149.62 | $165.33 |
| Turnaround time. | 26 to 60 days | 15 to 26 days | 8 to 12 days |
| Infinium Mouse Methylation. | $277.59 | $322.61 | N/A |
| Turnaround time. | 26 to 60 days | 15 to 26 days | N/A |
Notes
1. Pricing includes all costs for bisulfite conversion, microarrays, reagents, processing, and primary analysis. Service must be purchased in multiples of 8 (EPIC) or 12 (Mouse) samples. There is an additional charge for shipping the arrays.
2. Tiers: Turnaround time (TAT) is in business days. Availability for the Priority Tier is limited; contact [email protected] to see if this is a current option. More information about Pricing Tiers.
External Rates
| Service | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Infinium Human MethylationEPIC. | $300.70 | $348.36 | $383.73 |
| Turnaround time. | 26 to 60 days | 15 to 26 days | 8 to 12 days |
| Infinium Human Methylation Screening Array. | $145.28 | $180.96 | $197.07 |
| Turnaround time. | 26 to 60 days | 15 to 26 days | 8 to 12 days |
| Infinium Mouse Methylation. | $343.27 | $393.34 | N/A |
| Turnaround time. | 26 to 60 days | 15 to 26 days | N/A |
Notes
1. Pricing includes all costs for bisulfite conversion, microarrays, reagents, processing, and primary analysis. Service must be purchased in multiples of 8 (EPIC) or 12 (Mouse) samples. There is an additional charge for shipping the arrays.
2. Tiers: Turnaround time (TAT) is in business days. Availability for the Priority Tier is limited; contact [email protected] to see if this is a current option. More information about Pricing Tiers.
Submission
How to Order
Complete the DNA Plate Submission Form for submitting samples and email to [email protected].
Researchers should submit a minimum of 500 ng of DNA per sample at 25 ng/ul in a 96-well plate in a COLUMN-WISE fashion RANDOMIZING samples and keep two unique blank wells.
Samples can be dropped off at one of our campus locations:
1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
20 Snyder Hall (St. Paul campus)
If shipping samples, the following address should be used:
Please send the tracking information to [email protected].
UMN Genomics Center
ATTN: Nick Jones/ Jerry Daniel
3510 Hopkins Place N.
Building 4 Suite W402
Oakdale, MN 55128
612-625-7736
Deliverables
The client is emailed the GenomeStudio project file as well as the data in Excel.