Whole Genome Sequencing at the UMGC

Whole-genome sequencing (WGS) interrogates the entire DNA landscape, free from the compromises associated with targeted sequence capture (e.g., exome sequencing) or genotyping arrays. Thanks to falling costs and higher throughput from newer sequencing platforms, WGS is now an accessible first-line approach for the comprehensive analysis of human, animal, plant, model organisms, and microbes.

The UMN Genomics Center’s (UMGC) revamped WGS service centers on the ultra-high-throughput Ultima Genomics UG 100 platform, with the Element AVITI24 available where appropriate. The UG 100 produces single-end reads (≈200–300 bp) with exceptional throughput and a low cost per genome, providing excellent SNV accuracy and good indel quality for large-scale sequencing projects, while the AVITI24 delivers high-quality paired-end 2x150 bp reads for SNV and indel detection. The UMGC has extensively validated the UG 100, having sequenced over 2,000 human genomes at ≥ 30x coverage.

WGS Service Models

All-in-one package: Competitively priced, per-sample service that covers the full workflow: sample QC, library prep, sequencing, primary data QC, and for human or mouse, the data analysis of variants (VCFs) and summary reports. Coverage depth options for human or mouse include 30x, 60x, or 90x.

Human and Mouse WGS with data analysis
Non-human WGS
Model Organism WGS with data analysis

Customized coverage and special projects: Library prep and sequencing are priced separately for studies requiring ultra-deep sequencing, de novo assemblies, custom analysis, non-standard experimental designs, or accelerated timelines.

What’s Included

Sample Quality Control: Rigorous assessment of sample integrity and quantity.
Library Creation: PCR-free or PCR+ workflows, selected based on sample quality and project goals, to maximize coverage uniformity and minimize bias.
Next-Generation Sequencing: Sequencing is performed on either the UG 100 or AVITI24, delivering robust depth for genome-wide analysis.
Primary Data QC: Comprehensive evaluation of sequencing metrics to verify data completeness and quality prior to analysis.
Data Analysis: For human, mouse, and model organism projects, analysis includes mapping to the reference genome, variant analysis (including SNVs and indels), and, for model organisms, the identification of common transgenic insertions.

Getting Started

Combining Ultima’s high-throughput platform with the UMGC’s sequencing and informatics expertise provides a scalable WGS solution for population genomics, disease studies, and variant analysis. Contact [email protected] to get started.