The UMGC’s new Model Organism Sequencing Service (MOSS) offers cost-effective, high-throughput whole-genome sequencing for the workhorse model organisms Drosophila melanogaster and Caenorhabditis elegans, with other model organisms to be added in the coming months.
MOSS includes comprehensive support, from library preparation and sequencing to downstream bioinformatics analysis. Researchers receive a report with alignment files (BAMs) and variant call files (VCFs), as well as the identification and mapping of commonly used insertion elements, enabling seamless data interpretation.
Sequencing is performed at 30x coverage on the new Ultima UG 100 sequencer, providing a robust depth for genome analysis. Higher coverage options are available upon request.
The UMGC welcomes requests for other model organisms—contact us for details and a quote. For researchers who do not require analysis, we offer low-cost whole-genome sequencing on the UG 100 for any organism. View UG 100 pricing here.
Applications
By driving down the cost of sequencing, the UMGC aims to enable researchers to use MOSS as a standard, efficient means to accomplish a variety of analyses:
- Mutation and insertion mapping – Identify unknown genetic variants by comparing against reference strains.
- CRISPR edit confirmation – Validate genome edits with high precision.
- Structural variant detection – Characterize genomic changes such as deletions, duplications, and translocations.
- Genotyping and strain authentication – Ensure the accuracy of genetic backgrounds in research models.
Questions?
Contact Dr. Daryl Gohl at [email protected] with questions on model organism sequencing.
Pilot Phase Pricing:
- D. melanogster and C. elegans 30x whole genome sequencing and analysis will be available at a reduced rate:
- $25 per sample for gDNA submissions
- $50 per sample for whole organism submissions
- Please inquire about other organisms.
- Pricing is valid through June 30, 2025.
Sample Requirements:
- Minimum of 12 samples per submission.
- Samples must be submitted in multiples of 12.
Turnaround Time:
- Turnaround time during the pilot phase may vary, with results expected within approximately six weeks or less.
How to order
Samples can be submitted as either extracted genomic DNA (gDNA) (≥ 50 ng in 5 µL) or whole organisms. For specific submission guidelines and submission forms, please contact the UMGC at [email protected].
Samples can be dropped off at one of our campus locations
1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
20 Snyder Hall (St. Paul campus)
Samples can be shipped to the following address:
Please send the tracking information to [email protected].
UMN Genomics Center
ATTN: NGS Staff
3510 Hopkins Place N.
Building 4 Suite W402
Oakdale, MN 55128
612-625-7736