The Visium platform from 10x Genomics combines histology and spatially resolved whole transcriptome gene expression profiling to localize and quantify gene expression in the tissue context. The UMGC offers three assays for spatial analysis:
- Visium HD at single-cell resolution
- Visium HD 3’ at single-cell resolution
- Spatial Gene Expression (Visium Standard) at 55 uM resolution
Visium is compatible with a variety of tissues and species, depending on the specific assay you choose to employ:
- Species-agnostic: Visium Spatial Gene Expression and Visium HD 3’ for fresh frozen tissue leverages a poly(A)-based capture chemistry and is therefore species agnostic.
- Human and mouse: Visium CytAssist (HD and Spatial Gene Expression) is a probe-based assay that enables analysis of fresh frozen, fixed frozen, and FFPE tissue sections from human or mouse samples.
Explore the UMN Spatialomics Initiative website to learn more about the Visium platform and compare its technical details with those of other spatial platforms.
Questions
Contact [email protected] with questions on the 10x Visium platform or to schedule a consultation.
10x Genomics Visium projects can receive preferred pricing on Ultima UG 100 sequencing. Contact the UMGC at [email protected] for more information.