PacBio Revio Sequencing

The UMGC has determined that, for the time being, access to the PacBio Revio will be both faster and less expensive when outsourced, and we have partnered with reputable sequencing facilities to achieve this.

Thanks to recent advances in sequencing chemistry and adaptive loading, the SMRT Cell 25M with SPRQ typically generates ~ 15M raw reads with flexible sequencing run times of up to 24 hours, yielding up to 100-120 Gbp of HiFi data. As the sequencing polymerase makes successive passes along the same SMRT bell template, random errors present in each subread can be corrected to achieve high yields of >QV20 (99%) without sacrificing read length.

• Long Reads: With HiFi read lengths up to 25 kb, investigators can readily assemble complete genomes and sequence full-length transcripts. Long read lengths can resolve repetitive regions that are difficult for short-read technologies.
• High Accuracy: >99.999% consensus accuracy is achieved by sequencing the same molecule multiple times.
• Uniform Coverage: No bias based on GC content enables sequencing through regions inaccessible to other technologies. Template preparation and sequencing do not rely on amplification, thus there is no PCR bias for more uniform genome coverage.
• Epigenetics: With no PCR amplification step, base modifications are directly detected during sequencing without the need for bisulfite conversion.