Built on Single Molecule, Real-Time (SMRT) Sequencing technology, the PacBio Revio System delivers HiFi reads that are both exceptionally long (up to 30 kb) and highly accurate (>99.9%). This enables high-quality genome assemblies, structural variant detection, transcriptomics, isoform identification, and epigenetic analysis, while providing high consensus accuracy and uniform coverage.
The Revio system represents a significant advancement in long-read sequencing. The latest Revio 25M Cell with SPRQ chemistry increases HiFi data output by 33% while reducing input requirements compared to previous chemistries—enhancing efficiency and accessibility for researchers.
Highlighted applications
- Whole Genome Sequencing: de novo assembly, variant detection, structural variant detection
- Targeted Sequencing: amplicon sequencing for variant detection in specific regions and target capture
- Microbial Sequencing: combine ~5 Mb genomes in an 8-plex for single-digit contig assemblies
- RNA Sequencing: full-length transcripts for whole genome transcriptome annotation
- Complex Populations: full-length 16S, metagenomic profiling, shotgun metagenomic assembly
- Epigenetics: directly detects modifications during base incorporation
More information
Outsourcing
The UMGC has determined that, for the time being, access to the PacBio Revio will be both faster and less expensive when outsourced, and we have partnered with reputable sequencing facilities to achieve this.
Output
Thanks to recent advances in sequencing chemistry and adaptive loading, the SMRT Cell 25M with SPRQ typically generates ~ 15M raw reads with flexible sequencing run times of up to 24 hours, yielding up to 100-120 Gbp of HiFi data. As the sequencing polymerase makes successive passes along the same SMRT bell template, random errors present in each subread can be corrected to achieve high yields of >QV20 (99%) without sacrificing read length.
Benefits
- Long Reads: With HiFi read lengths up to 25 kb, investigators can readily assemble complete genomes and sequence full-length transcripts. Long read lengths can resolve repetitive regions that are difficult for short-read technologies.
- High Accuracy: >99.999% consensus accuracy is achieved by sequencing the same molecule multiple times.
- Uniform Coverage: No bias based on GC content enables sequencing through regions inaccessible to other technologies. Template preparation and sequencing do not rely on amplification, thus there is no PCR bias for more uniform genome coverage.
- Epigenetics: With no PCR amplification step, base modifications are directly detected during sequencing without the need for bisulfite conversion.
Starting a Project
The UMGC provides comprehensive, end-to-end PacBio services, including rigorous sample QC, library preparation, and advanced data analysis. We are committed to delivering high-quality, long-read sequencing solutions to support cutting-edge genomic research. Contact us at [email protected] to learn how our PacBio Revio services can advance your studies.
UMN Rates
| Library Creation | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Amplicon. No Shearing/Size Selection. | $519.72 | $789.45 | $1,292.68 |
| Turnaround time. | 10 - 15 days | 5 - 10 days | 2 - 4 days |
| HiFi. TMK 3.0 Microbial Multiplexing. | $460.27 | $644.51 | $960.42 |
| Turnaround time. | 12 - 21 days | 7 - 15 days | 3 - 7 days |
| HiFi. TMK 3.0. Includes Library QC. | $849.32 | $1,296.72 | $1,854.68 |
| Turnaround time. | 13 - 21 days | 7 - 14 days | 3 - 7 days |
| IsoSeq.TMK 3.0. Includes Library QC. | $614.31 | $954.31 | $1,272.35 |
| Turnaround time. | 12 - 22 days | 7 - 13 days | 4 - 7 days |
| Sequencing | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Revio SMRT Cell 25M. Outsourced Run. | $2,174.01 | $2,704.80 | N/A |
| Turnaround time. | 3 - 10 days | 3 - 6 days | N/A |
Notes
Tiers: Tiers: Turnaround time (TAT) is in business days. Availability for the Priority Tier is limited; contact [email protected] to see if this is a current option. More information about Pricing Tiers.
External Rates
| Library Creation | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Amplicon. No Shearing/Size Selection. | $642.66 | $942.26 | $1,501.19 |
| Turnaround time. | 10 - 15 days | 5 - 10 days | 2 - 4 days |
| HiFi. TMK 3.0 Microbial Multiplexing. | $569.16 | $773.92 | $1,124.81 |
| Turnaround time. | 12 - 21 days | 7 - 15 days | 3 - 7 days |
| HiFi. TMK 3.0. Includes Library QC. | $1,050.25 | $1,547.66 | $2,167.48 |
| Turnaround time. | 13 - 21 days | 7 - 14 days | 3 - 7 days |
| IsoSeq.TMK 3.0. Includes Library QC. | $759.65 | $1,137.42 | $1,490.76 |
| Turnaround time. | 12 - 22 days | 7 - 13 days | 4 - 7 days |
| Sequencing | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Revio SMRT Cell 25M. Outsourced Run. | $2,688.30 | $3,277.25 | N/A |
| Turnaround time. | 3 - 10 days | 3 - 6 days | N/A |
Notes
Tiers: Tiers: Turnaround time (TAT) is in business days. Availability for the Priority Tier is limited; contact [email protected] to see if this is a current option. More information about Pricing Tiers.
Submission
How to Order
- Please contact [email protected] for project specifications.
- After project details are finalized, complete PacBio Sample Submission Form and email to [email protected].
Samples can be dropped off at any of our campus locations
- 1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
- 20 Snyder Hall (St. Paul campus)
Shipping Instructions
Please give advance notice of your sample submission date and time so staff can be prepared to receive samples. If shipping samples from outside the University of Minnesota, ship via express shipping carrier to the address below.
Please send the tracking information to [email protected].
UMN Genomics Center
ATTN: Elyse Froehling
3510 Hopkins Place N.
Building 4 Suite W402
Oakdale, MN 55128
612-625-7736
Deliverables
Data Release
There are four options for transferring data from the UMGC to clients: 1) delivery to the Minnesota Supercomputing Institute’s (MSI) high-performance file system, 2) download from a secure website, 3) download with Globus, or 4) shipment on an external hard drive. Please indicate your data delivery preference when placing an order for sequencing.
1. MSI storage
Internal clients have their data released to MSI's Shared User Resource Facility Storage (SURFS). Delivered data will be located in the "data_delivery" folder in your group's folder on MSI's primary filesystem (home/GROUP/data_delivery/umgc). MSI does not charge for SURFS storage costs, but files expire and are removed one year after they've been delivered. Files should be copied to other MSI storage locations such as Tier2, Tier3, or your group's "shared" folder before they expire.
2. Web download
Internal clients that opt-out of MSI storage and external clients can download their data from a secure website using either a web browser or a command-line download tool, complete instructions are provided in an email from the UMGC. The client’s data is available for download for 30 days, after which the data will be removed from the data download website and the client takes responsibility for storing the data.
3. Globus
Internal and External clients can use Globus to download their data. This is the recommended method for external clients to download large datasets.
4. Hard drive
External clients may have data shipped on a hard drive purchased by the UMGC and invoiced to the client at a cost of $250 per hard drive.
Data Recovery
The UMGC archives most customer data for a year and some datasets are retained for 5 years or more. If you need a dataset re-delivered email a request to [email protected] to initiate data recovery. The UMGC does not provide any guarantee that data can be successfully recovered from the archive.