Our staff can isolate total RNA from blood, cells or tissues by methods appropriate to your project. Extracted RNA can also be returned to the client when requested (shipping charges apply). RNA is extracted from client samples by appropriate methods, either TRIzol or column-based extraction, depending on sample type, client choice and end-use.
For example, clients wishing to profile miRNA expression would be advised to use a TRIzol type extraction because it preserves the small RNA fraction that contains miRNAs better than column methods. Please contact us with any questions or requirements. Initial assessment of RNA purity and concentration is performed by NanoDrop spectrophotometer analysis and RiboGreen Assay, which is included in the service. RNA integrity is determined using the Agilent 2100 Bioanalyzer, TapeStation or Caliper GX (optional but highly recommended).
UMGC takes special care of RNA samples and only performs manual extractions due to the high sensitivity of RNA. We run RNA-based downstream projects in our own facility and use extraction methods to provide quality RNA that meets our application needs. UMGC can offer complete workflows of cells to data.
We routinely offer RNA extraction services from the following sources. If you have other sources not listed below, please contact us.
- Paxgene Samples
- Buccal Swabs/Saliva
- FFPE
- Cell Pellets
- Plant and Animal Tissue
- Environmental and Fecal
- Bacteria
The assessment of RNA purity and concentration by UV spectroscopy (NanoDrop) and fluorimetry (RiboGreen quantification). RNA integrity is determined using the Agilent 2100 Bioanalyzer or TapeStation.
Questions?
Contact: [email protected]
UMN Rates
| Service | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Qiagen RNeasy Plus Universal. | $58.05 | $77.47 | $105.70 |
| Turnaround time. | 10 - 23 days | 6 - 9 days | 4 - 5 days |
| Volume. | 8 - 96 samples | 8 - 48 samples | 8 - 24 samples |
| Qiagen RNeasy HTP. | $40.98 | $58.23 | $91.56 |
| Turnaround time. | 17 - 26 days | 7 - 11 days | 5 - 6 days |
| Volume. | 24 - 384 samples | 24 - 192 samples | 24 - 96 samples |
| Qiagen RNeasy Plant. | $57.58 | $79.93 | $111.10 |
| Turnaround time. | 10 - 23 days | 6 - 9 days | 4 - 5 days |
| Volume. | 8 - 96 samples | 8 - 48 samples | 8 - 16 samples |
| Invitrogen PureLink FFPE. | $51.56 | $70.30 | $97.86 |
| Turnaround time. | 8 - 18 days | 5 - 7 days | 3 - 4 days |
| Volume. | 8 - 96 samples | 8 - 48 samples | 8 - 24 samples |
| PAXgene Blood. | $62.45 | $85.91 | $118.19 |
| Turnaround time. | 12 - 26 days | 7 - 11 days | 5 - 6 days |
| Volume. | 8 - 96 samples | 8 - 48 samples | 8 - 16 samples |
Notes:
All RNA extraction services include quantification and sizing. HTP = High throughput.
Tiers: More information about Pricing Tiers. Turnaround time is in business days. Availability for the Priority Tier is limited. Sample sizes beyond the maximum volume listed for Savings Tier will qualify for Scale Tier pricing. Contact [email protected] for details.
External Rates
| Service | Savings Tier | Speed Tier | Priority Tier |
|---|---|---|---|
| Qiagen RNeasy Plus Universal. | $71.37 | $92.96 | $124.33 |
| Turnaround time. | 10 - 23 days | 6 - 9 days | 4 - 5 days |
| Volume. | 8 - 96 samples | 8 - 48 samples | 8 - 24 samples |
| Qiagen RNeasy HTP. | $50.39 | $69.56 | $106.59 |
| Turnaround time. | 17 - 26 days | 7 - 11 days | 5 - 6 days |
| Volume. | 24 - 384 samples | 24 - 192 samples | 24 - 96 samples |
| Qiagen RNeasy Plant. | $70.79 | $95.65 | $130.27 |
| Turnaround time. | 10 - 23 days | 6 - 9 days | 4 - 5 days |
| Volume. | 8 - 96 samples | 8 - 48 samples | 8 - 16 samples |
| Invitrogen PureLink FFPE. | $63.40 | $84.24 | $114.86 |
| Turnaround time. | 8 - 18 days | 5 - 7 days | 3 - 4 days |
| Volume. | 8 - 96 samples | 8 - 48 samples | 8 - 24 samples |
| PAXgene Blood. | $76.78 | $102.88 | $138.72 |
| Turnaround time. | 12 - 26 days | 7 - 11 days | 5 - 6 days |
| Volume. | 8 - 96 samples | 8 - 48 samples | 8 - 16 samples |
Notes:
All RNA extraction services include quantification and sizing. HTP = High throughput.
Tiers: More information about Pricing Tiers. Turnaround time is in business days. Availability for the Priority Tier is limited. Sample sizes beyond the maximum volume listed for Savings Tier will qualify for Scale Tier pricing. Contact [email protected] for details.
Before submitting samples for extraction, please contact [email protected] for project specifications.
- Guidelines on sample submission.
- Complete the sample submission form and email to [email protected]
Campus drop-off locations:
- 1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
- 20 Snyder Hall (St. Paul campus)
Shipping information:
RNA is susceptible to degradation; therefore, it is recommended that samples sent for RNA extraction should be preserved using RNAlater or similar product, flash-frozen with liquid nitrogen and shipped on dry ice to ensure that they remain frozen until extraction is performed.
UMN Genomics Center
ATTN: Jerry Daniel/Veronica Tonnell
3510 Hopkins Place N.
Building 4 Suite W402
Oakdale, MN 55128
612-626-9167
Please send the tracking information to [email protected].