RNA Extraction


Overview

Our staff can isolate total RNA from blood, cells or tissues by methods appropriate to your project. Extracted RNA can also be returned to the client when requested (shipping charges apply). RNA is extracted from client samples by appropriate methods, either TRIzol or column-based extraction, depending on sample type, client choice and end use. For example, clients wishing to profile miRNA expression would be advised to use a TRIzol type extraction because it preserves the small RNA fraction that contains miRNAs better than column methods. Please contact us with any questions or requirements. Initial assessment of RNA purity and concentration is performed by NanoDrop spectrophotometer analysis and RiboGreen Assay which is included in the service. RNA integrity is determined using the Agilent 2100 Bioanalyzer, TapeStation or Caliper GX (optional but highly recommended).

UMGC takes special care of RNA samples and only perform manual extractions due to high sensitivity of RNA. We run RNA-based downstream projects in our own facility and use extraction methods to provide quality RNA that meets our application needs. UMGC can offer complete workflows of cells to data.

UMGC also offers DNA Extraction.

We routinely offer RNA extraction services from following sources. If you have other sources not listed below, please contact us.

  • Paxgene Samples
  • Buccalk Swabs/Saliva
  • FFPE
  • Cell Pellets
  • Plant and Animal Tissue
  • Environmental and Fecal
  • Bacteria

The assessment of RNA purity and concentration by UV spectroscopy (NanoDrop) and fluorimetry (RiboGreen quantification). RNA integrity is determined using the Agilent 2100 Bioanalyzer or TapeStation.

Questions?


Contact Dinesha Walek: walek001@umn.edu

Pricing

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Standard

Service Scale Unit UMN External
Qiagen RNeasy Plus Universal. 1-any sample $28.50 $32.81
Qiagen RNeasy Plant. 1-any sample $27.55 $31.72
PAXgene Blood. 1-any sample $33.59 $38.64
FFPE Samples. RNeasy. 1-any sample $29.88 $34.40
GLOBINclear mRNA Reduction. 1-any sample $32.82 $37.72

 

Notes:
  1. All RNA extraction service include quantification.
Guidelines

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Submission

How to Order


Project initiation, please contact extract@umn.edu for project specifications.

Please review our nucleic acid extraction sample submission guidelines. Once project details are finalized, complete the nucleic acid extraction sample submission form and email to extract@umn.edu.

Samples can be dropped-off at one of our laboratory locations.

  • 1-202 Nils Hasselmo Hall (Minneapolis campus)
  • 1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)
  • 20 Snyder Hall (St. Paul campus)
If shipping samples, the following address should be used.

Please send the tracking information to extract@umn.edu.

University of Minnesota Genomics Center
Attn: Dinesha Walek/Jiangming Deng
312 Church Street SE
1-202 NHH
Minneapolis, MN 55455
612-624-3177

Shipping conditions

RNA is susceptible to degradation; therefore, it is recommended that samples sent for RNA extraction should be preserved using RNAlater or similar product, flash frozen with liquid nitrogen and shipped on dry ice to ensure that they remain frozen until extraction is performed.

Deliverables

Client receives a QC report with sample details.

FAQ

What is the turnaround time?


It depends upon sample numbers. We handle both small (1-2 samples) and large Please contact us for a timeline.

What is your recommendation on shipping?


  • Dry Ice–Cells, Tissue, Blood, Fecal and Plant
  • RNA is susceptible to degradation; therefore, it is recommended that samples sent for RNA extraction should be preserved using RNAlater or similar product, flash frozen with liquid nitrogen and shipped on dry ice to ensure that they remain frozen until extraction is performed.

What is your shipping address?


Attn: Dinesha Walek/Jiangming Deng
312 Church Street SE
1-202 NHH
Minneapolis, MN 55455

How do you determine quality of extracted RNA?


The assessment of RNA purity and concentration is by both UV spectroscopy (NanoDrop) and fluorimetry (PicoGreen quantification). RNA integrity is determined using the Agilent 2100 Bioanalyzer or TapeStation.

What type extractions do you offer?


We use a wide range of commercially available kits, and bead beating methods, listed below are some common methods. If you have your own method please contact us.

  • QIAGEN RNeasy Bloold and Tissue Kit: a standard, lower-cost silica-binding purification method that provides RNA of excellent quality for genomic applications.
  • ORAGENE BUCCAL: Oragene buccal cell collection kits offer pain-free and noninvasive collection that is readily self-administered, suitable for shipment in standard mail, and stable at room temperature.
  • QIAGEN RNeasy Plus Universal Plus Kit:
  • THERMO RiboPure Kit:
  • PAXgene:
  • QIAGEN MagAttract PowerMicrobiome DNA/RNA: a “bead-beating” method that uses abrasive beads to break the cell walls of microbes in stool and other samples. Extracts both RNA and DNA This service is typically used in combination with 16S/18S/Std NGS Seq sequencing.

How much starting material do you need?


You can send ~ 50–100 mg of plant leaf tissue, about 40 mg of Human/Animal Tissue and ideally 1X 106 Cells. These are suggested amounts, we can extract other smaller and larger amounts.

Can you Store our samples?


UMGC has the capability of archiving your sample with 2D-Barcoded tubes/Plates and a retrieval system. This also enables us to re-rack/cherry pick samples for future downstream applications at the UMGC or ship them to an off-site location.