Sequence Capture

Sequence capture is used to enrich genomic regions of interest, thereby reducing the sequencing depth (and cost) needed to obtain a given coverage level. Capture baits are oligonucleotides that are used to pull-down complementary DNA through hybridization. Both custom and pre-designed capture baits are available from several vendors (listed in the Vendor Options tab), for a variety of applications.

Exome sequencing

Exome sequencing is a common application of sequence capture that enriches for exons, the coding region of expressed genes. As an example, the human exome constitutes about 1% of the total genome size. A variety of pre-defined exome panels, or expanded exome panels, are available from Agilent, Roche (NimbleGen), and Illumina.

Custom designs

While many off-the-shelf options exist, it is often desirable or necessary to develop a custom capture for a specific target or organism. We have significant experience working with vendors to develop baits for these custom projects. We can coordinate and carry-out your entire project, from the design phase through data delivery. Please inquire.

Costs

Sequence capture projects include two costs:

1. Library creation (labor and generic reagents)
2. Application-specific capture reagents, including baits and vendor-specific library creation reagents.

Questions?

Contact: next-gen@umn.edu.