The MACSQuant Tyto by Miltenyi is a next-generation, benchtop cell sorter equipped with 3 lasers, which allows for 10-parameter cell sorting. The sorting process occurs exclusively within the disposable, fully closed MACSQuant Tyto Cartridge, which keeps samples contamination-free and prevents aerosol and droplet formation. Unlike during conventional droplet sorting procedures, cells do not experience high pressure, they are not decompressed, and no charge is applied.
The UMGC offers the MACSQuant Tyto as a DIY instrument, giving researchers more options for flow-based collection, either upstream of UMGC analytical services or simply as a standalone flow cytometry service. Common applications are preparations of cell suspensions for single-cell or bulk RNA-seq workflows.
Training
At the required 4-hour training session, a UMGC scientist will guide a researcher through a "real" sort on the Tyto instrument, using viable cells provided by the researcher. The UMGC will train on instrument operation, maintenance, and clean-up protocols, and the researcher will be responsible for gating decisions, antibodies, etc. for the "real" sort training session.
Reserving the Tyto
- Reservations are for a fixed 2-hour DIY session at $177.41/session.
- Timeslot options are Monday - Friday either 9:00 am - 11:00 am or 11:00 am - 1:00 pm to enable two users per day.
- The first scheduled reservation needs to be within 6 months of training.
- Tyto location: 3-230E CCRB
Contact single-cell@umn.edu to reserve a 2-hour timeslot.
Purchasing a Cartridge
The UMGC purchases the Tyto cartridges in bulk and will provide individual cartridges to researchers at a cost. Researchers are not permitted to bring their own cartridges.
Questions
For additional questions on using the Tyto, setting up training, or reserving the instrument, contact single-cell@umn.edu.
| Service | Scale | Unit | UMN | External |
|---|---|---|---|---|
| Required training. | ||||
| A staff scientist will guide new users on Tyto sorting using actual cells in a 4-hour session. Includes cartridge. | 1 | session | $550.85 | $702.69 |
| Client-operated 2-hour session. | ||||
| Client independently uses the Tyto instrument in 3-230E CCRB to fully execute their own cell sort. Rate includes instrument usage. Cartridge rate is not included. | 1 | session | $177.41 | $226.31 |
| Client-operated 4-hour session. | ||||
| Client independently uses the Tyto instrument in 3-230E CCRB to fully execute their own cell sort. Rate includes instrument usage. Cartridge rate is not included. | 1 | session | $354.82 | $452.62 |
| MACSQuant Tyto Cartridges. | ||||
| Cartridges are purchased through the UMGC. Users are not permitted to bring their own cartridges. | 1 | cartridge | $170.46 | $217.44 |
Location
Location for Tyto training and operation
Room: 3-230E
Building: Cancer and Cardiovascular Research Building
Contact: single-cell@umn.edu
Phone: 612-624-6269
Technical Specifications
- Maximum loading cell number 5×10^7 cells/mL
- Laser excitation 405 nm, 100 mW | 488 nm, 100 mW | 638 nm, 100 mW
- Emission detectors:
- Channel: Filter:
- V1, V2 450/50 nm, 525/50 nm
- B1, B2, B3, B4 525/50 nm, 585/40 nm, 655–730 nm, 750 nm Long-Pass
- R1, R2 655–730 nm, 750 nm Long-Pass
- Backscatter and side scatter for detection of size and granularity of cells