Single Cell 3’ Gene Expression Assay
Provides single-cell transcriptome 3' gene expression and multi-omic capabilities to profile tens of thousands of cells. Explore cellular heterogeneity, novel targets, and biomarkers with combined gene expression, surface protein expression, or CRISPR edits in each cell.
- Captures 100-20,000 cells/sample and up to 160,000 cells/run
- Optional Feature Barcoding method for cell surface protein
- Minimum sequencing depth recommended by 10x Genomics Sequencing Requirements for 3' Gene Expression (some projects may require increased sequencing)
- Cell Preparation Guide and demonstrated protocols
- Explore 10x’s Gene Expression publications, sample guides, and grant writing support.
Single Cell 5’ Immune Profiling Assay
Capable of modular integration of multiple data types, including mRNA-based whole transcriptome or targeted gene expression, immunophenotyping, adaptive immune repertoire clonality and diversity, and antigen specificity, simultaneously from the same single cells.
- Efficiently partition 500–20,000 cells/pool for up to 160,000 cells per run
- Optional Feature Barcoding method for cell surface protein
- V(D)J Enrichment for human or mouse T or B cell
- Minimum sequencing depth recommended by 10x Genomics for Immune Profiling - some projects may require increased sequencing
- Cell Preparation Guide and demonstrated protocols
- Explore 10x’s Immune Profiling publications, resources, and sample guides.
On-Chip Multiplexing for 3’ and 5
Allows sample multiplexing of up to four samples with corresponding gel beads.
- Efficiently partition either 500-5,000 cells per sample or 40,000 cells per chip
- Compatible with either GEM-X Universal 3'v4 and 5'v3 Gene Expression
- Batch and multiplex up to 8 samples per chip without the need for sample Hashing
- Ideal for lower sample input
- Reduced reagent consumption and lower overall costs, providing a more cost-effective entry point into single-cell workflows
Single Cell Gene Expression Flex
Provides scalable solutions to measure gene expression of 18,000+ protein-coding genes and multi-omics capability by fixing, storing, and batching of samples for a later run date.
- Efficiently partition either 500-20,000 cells for Singleplex or Multiplex 80,000- 2.56 million cells per run
- Singleplex: Allows barcoding of 4 samples with barcode probes and individual captures
- Multiplex: Available as either 4- or 16-plex kit allowing to capture either a maximum of 4 or 16 samples or 80,000 cells to 320,000 cells per GEM reaction, with each kit carrying gel beads for 4 GEM reactions
- Enables larger single-cell projects by allowing for higher cell counts per sample or up to 256 samples.
- Flexibility of capturing more cells per sample
- Pre-designed panel for Human and Mouse with customizable panels for additional species.
- Ideal for fixed samples, low-quality, or degraded RNA
- Optional multiomic capability, including Cell surface protein, intracellular protein, or CRISPR screening.
- Compatible with diverse sample types, including freshly dissociated tissue, frozen tissue, PFA-fixed tissues, primary cells, cell lines, nuclei, and FFPE tissue
Single Cell Multiome ATAC + Gene Assay
Analyze open chromatin accessibility and gene expression at the single-cell level to provide insights into the regulatory landscape of chromatin and transcriptome heterogeneity. Uncover gene regulatory networks and gain deeper insights into cell types and states by linking open chromatin with gene expression at single-cell resolution.
- Efficiently partition 500–10,000+ nuclei/channel, for up to 80,000 nuclei per run
- Integrate scATAC-Seq data with scRNA-Seq data using Seurat v3
- Minimum sequencing depth recommended by 10x Genomics for Single Cell ATAC and Multiome - some projects may require increased sequencing
- Sample prep protocols