SARS-CoV-2 Strain Sequencing
The UMGC has developed a PCR amplicon-based SARS-CoV-2 strain sequencing service that leverages protocols developed by the ARTIC Network, while reducing cost and increasing throughput. The details of our methodology have been published here. In brief, the method employs 95 PCR primers pairs to tile the SARS-CoV-2 genome with overlapping amplicons in a total of four multiplexed amplification reactions to achieve virtually complete genomic assembly.
We are offering this service to both UMN researchers and external researchers at cost recovery rates, in order to help accelerate the fight against the COVID-19 pandemic. The service is a “soup-to-nuts” workflow (including QC, library creation, sequencing, genomic assembly and annotation relative to reference, and (optional) upload to NextStrain.
Please see the service page for more information and pricing.