Agenda and Registration
Agenda
8:30 AM - Check-in and light refreshments (no breakfast)
9:00 AM - Welcome and intro, Christine Henzler, PhD, MSI Co-Director of Research Informatics
9:10 AM - Xavier Revelo, PhD, Associate Professor, Integrative Biology & Physiology and Center for Immunology
9:45 AM - Jop van Berlo, PhD, Associate Professor, Lillehei Heart Institute
10:20 AM - Coffee break
10:40 AM - Zachary Myers, PhD, Postdoctoral Researcher (Greenham Lab), Department of Plant and Microbial Biology
11:15 AM - Christine Henzler, PhD, MSI Co-Director of Research Informatics
11:35 AM - Kenny Beckman, PhD, UMN Genomics Center Director
12:00 PM - UMGC CoLab open house
12:30 PM - Adjourn
Registration
This event is for UMN researchers. Advanced registration is required for this in-person symposium, and there is no cost to attend.
Abstracts
Single-cell genomics to study adaptive immune responses in metabolic disease
Xavier Revelo, PhD, Associate Professor, Integrative Biology & Physiology and Center for Immunology
Single-cell genomic technologies have revolutionized molecular biology by expanding our abilities to gain novel insights into inflammatory disease. Notably, inflammation plays a crucial role in the initiation and progression of metabolic disorders associated with obesity. However, our understanding of the mechanisms orchestrated by resident and infiltrating immune cells is limited. Using single-cell RNA and repertoire sequencing, we have discovered a bias towards aberrant inflammatory B and T helper subsets in the liver and gut-associated lymphoid tissues in a mouse model of disease. Overall, single-cell approaches at the interface of immunology and metabolism have been instrumental in identifying key mechanisms of metabolic disease.
Single-cell sequencing of cardiac cells using different platforms
Jop van Berlo, PhD, Associate Professor, Lillehei Heart Institute
Circadian cross-clock: using single-cell technologies to study rhythmic gene expression across cell types in plants
Zachary Myers, PhD, Postdoctoral Researcher (Greenham Lab), Department of Plant and Microbial Biology
The development and application of single-cell genomics approaches have enabled an unprecedented level of resolution to explore biology. While these techniques are of great interest to many plant biologists, they are often designed around and built with non-plant models in mind. Despite these challenges, the Greenham lab has begun adopting single-cell approaches to study circadian rhythms in gene expression at cellular resolution. In this presentation I will highlight how this level of resolution allows us to approach new questions surrounding cell-cell communication between oscillators, and will share key lessons learned along the way for researchers considering applying these approaches.
Leveraging the CoLab for single-cell genomics
Kenny Beckman, PhD, UMN Genomics Center Director
The UMGC CoLab is a new lab environment designed and equipped to help users succeed in complex genomic NGS workflows such as single-cell genomics (SCG). By providing specialized instrumentation, advanced training, and full 24/7 access, the CoLab enables faster turnaround, more affordable service, and convenience. We have begun acquiring cell isolation and purification devices and in June will begin the CoLab "Demo Days" program, in which not-yet-purchased devices will be placed in the core for extended periods for user "beta testing" in advance of a purchasing decision. Come hear about the S2 Genomics Singulator 200 (automated tissue disruption), the Miltenyi MACSQuant Tyto Cell Sorter (gentle cell sorting), the LevitasBio Levicell EOS (purification of viable cells and nuclei). All of these devices will be available starting in June, and will enable higher-quality SCG analysis using downstream workflows including 10X Genomics, Parse, and others.
Location and Parking
Location:
The Single Cell Symposium is hosted in the Cancer and Cardiovascular Research Building (2231 6th St SE, Minneapolis, MN 55455) located on the East Bank of the University of
Minnesota. Check-in will be available at the main entrance.
Parking:
For parking near CCRB, we suggest parking in the Maroon and Victory daily lots ($6/day without in/out privileges) located across the street.
Additional transportation options:
The Metro Green Line (Light Rail) and select campus buses have stops near CCRB. A variety of detailed campus maps can help you navigate to CCRB.
About the Cores
UMN Genomics Center
The University of Minnesota Genomics Center (UMGC) provides genomic technologies and services to researchers and clinicians at the University of Minnesota, and to external academic and industry scientists throughout the United States and internationally. The UMGC acquires state-of-the-art instrumentation and offers an array of services, including next-generation sequencing, expression analysis, genotyping, single-cell and spatial genomics, metagenomics, and related support services such as nucleic acid extraction and quality control.
Minnesota Supercomputing Institute
The Minnesota Supercomputing Institute (MSI) provides advanced research computing infrastructure and expertise to the University of Minnesota research and scholarly community and the State of Minnesota in order to advance and accelerate research and foster innovation and discoveries through advanced computing technologies, scientific computing, and informatics, application development, and services.
