NextSeq 2000 Sequencing


Overview
hires-nextseq2000-front copy

The NextSeq 2000 is Illumina’s newest platform that uses advancements in optics, instrument design, and 2-channel chemistry to increase output. This new sequencing technology makes it possible for researchers to sequence a range of conventional and emerging applications by increasing throughput while cutting down on costs per run when compared to its predecessor, the NextSeq 550.

The result is a highly flexible and scalable benchtop system that offers the highest cluster density flow cell of any on-market NGS system to date. It drives down the cost per gigabase making it more accessible for researchers.

As a high-throughout instrument, the NextSeq 2000 is a scalable platform that supports more complex, informative studies and a broad range of standard NGS methods, as well as emerging techniques, including spatialomics. 

This instrument expands the UMGC’s comprehensive sequencing portfolio by replacing the NextSeq 550 as the instrument of choice for projects needing a fast turnaround and increased flexibility.

Output


Currently, there are two types of flow cell options, the P2 and P3, available with a range of outputs to accommodate a wide variety of NGS projects. The P3 is a high-output flow cell that generates up to 1 billion reads per run, more than nearly three times the previously available NextSeq 550.  

The lower-throughput P1 flow cell is expected to be released in the second half of 2021 and will have an output between 20-40 Gb making it an attractive option for MiSeq and NextSeq 550 users. 

Benefits


The primary advantages of the NextSeq 2000 are the sequencing speed and multiple run configurations that enable sequencing outputs of 36 - 300 Gb per run. 

Four run configurations are available: the 100-cycle kit, the 200-cycle kit, the 300-cycle kit, and the 50-cycle kit, which is available in the P3 only. The added 50-cycle kit targets infectious disease, small RNA, and spatial transcriptomics applications. 

In addition, users have the benefit of not needing to wait to fill a large, multi-lane flow cell (NovaSeq) before data can be generated. Whether it is the P2 or P3 flow cell, researchers purchase the full NextSeq flow cell (no lane splitting) and sequencing will begin once libraries are complete and the instrument is available. 

Drawbacks


Due to the 2-channel chemistry, the NextSeq does not tolerate low diversity libraries as well as the MiSeq 4-channel platform, and in order to generate high-quality data, a higher spike-in concentration of a diverse control library is required. Thus, amplicon libraries with low sequence diversity should be designed with phasing bases to frameshift low diversity regions.

In addition, output capacity for custom libraries, including amplicon libraries and low diversity applications, may generate up to 25% less data in an effort to improve data quality. 

Questions?


Please contact Elyse Cooper or Aaron Becker at next-gen@umn.edu for further details on the NextSeq platform.

Pricing

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2022 Standard Rates

Service Scale Unit UMN External
NextSeq P2 Flow Cell - 360 M reads/lane.
100 cycle (50 PE or 100 SR). 1 1-any lane $2,378.09  $2,734.80 
200 cycle (100 PE). 2 1-any lane $4,236.67  $4,872.17 
300 cycle (150 PE). 3 1-any lane $5,249.30  $6,036.70 
NextSeq P3 Flow Cell - 1 B reads/lane.
50 cycle (25 PE or 50 SR). 4 1-any lane $3,502.20  $4,027.52 
100 cycle (50 PE or 100 SR). 5 1-any lane $4,856.54  $5,585.02 
200 cycle (100 PE). 6 1-any lane $6,549.47  $7,531.90 
300 cycle (150 PE). 7 1-any lane $8,580.99  $9,868.14 
         
Note:

Prices are valid starting January 1, 2022. In an effort to improve data quality, the output capacity for custom libraries, including amplicon libraries and low diversity applications, may generate up to 25% less data due to the addition of PhiX.

Ouput:
  1. Output ≥ 360,000,000 reads x 100 bp = 36 Gb
  2. Output ≥ 360,000,000 reads x 200 bp = 72 Gb
  3. Output ≥ 360,000,000 reads x 300 bp = 108 Gb
  4. Output ≥ 1,000,000,000 reads x 50 bp = 50 Gb
  5. Output ≥ 1,000,000,000 reads x 100 bp = 100 Gb
  6. Output ≥ 1,000,000,000 reads x 200 bp = 200 Gb
  7. Output ≥ 1,000,000,000 reads x 300 bp = 300 Gb

2021 Standard Rates

Service Scale Unit UMN External
NextSeq P2 Flow Cell - 360 M reads/lane.
100 cycle (100 SR or 50 PE). 1 1-any lane $1,888.69 $2,362.57
200 cycle (100 PE). 2 1-any lane $3,400.40 $4,253.56
300 cycle (150 PE). 3 1-any lane $4,224.04 $5,283.86
NextSeq P3 Flow Cell - 1 B reads/lane.
50 cycle (50 SR or 25 PE). 4 1-any lane $2,803.00 $3,506.28
100 cycle (100 SR or 50 PE). 5 1-any lane $3,904.58 $4,884.25
200 cycle (100 PE). 6 1-any lane $5,281.56 $6,606.70
300 cycle (150 PE). 7 1-any lane $6,933.93 $8,673.64
         
Note:

Prices are valid until December 31, 2021. In an effort to improve data quality, the output capacity for custom libraries, including amplicon libraries and low diversity applications, may generate up to 25% less data due to the addition of PhiX.

Ouput:
  1. Output ≥ 360,000,000 reads x 100 bp = 36 Gb
  2. Output ≥ 360,000,000 reads x 200 bp = 72 Gb
  3. Output ≥ 360,000,000 reads x 300 bp = 108 Gb
  4. Output ≥ 1,000,000,000 reads x 50 bp = 50 Gb
  5. Output ≥ 1,000,000,000 reads x 100 bp = 100 Gb
  6. Output ≥ 1,000,000,000 reads x 200 bp = 200 Gb
  7. Output ≥ 1,000,000,000 reads x 300 bp = 300 Gb
Guidelines

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Submission

How to order


Researchers can download the appropriate submission form for submitting samples or submitting client-made libraries. Once the form has been completed, please submit the submission by emailing next-gen@umn.edu.

Forms

Illumina Sequencing Request Form (Libraries)

Illumina Sequencing Request Form (Samples)

Samples can be dropped-off at one of our laboratory locations.

1-202 Nils Hasselmo Hall (Minneapolis campus)

1-210 Cancer & Cardiovascular Research Building (Minneapolis campus)

20 Snyder Hall (St. Paul campus)

If shipping samples, the following address should be used.

Please send the tracking information to next-gen@umn.edu.

University of Minnesota Genomics Center
1475 Gortner Ave.
28 Snyder Hall
St. Paul, MN 55108
612-625-7736

Deliverables

Data Release


There are four options for transferring data from the UMGC to clients: 1) delivery to the Minnesota Supercomputing Institute’s (MSI) high-performance file system, 2) download from a secure website, 3) download with Globus, or 4) shipment on an external hard drive. Please indicate your data delivery preference when placing an order for sequencing.

1. MSI storage

Internal clients have their data released to MSI's Shared User Resource Facility Storage (SURFS). Delivered data will be located in the "data_delivery" folder in your group's folder on MSI's primary filesystem (home/GROUP/data_delivery/umgc). MSI does not charge for SURFS storage costs, but files expire and are removed one year after they've been delivered. Files should be copied to other MSI storage locations such as Tier2, Tier3, or your group's "shared" folder before they expire. 

2. Web download

Internal clients that opt-out of MSI storage and external clients can download their data from a secure website using either a web browser or a command-line download tool, complete instructions are provided in an email from the UMGC. The client’s data is available for download for 30 days, after which the data will be removed from the data download website and the client takes responsibility for storing the data.

3. Globus

Internal and External clients can use Globus to download their data. This is the recommended method for external clients to download large datasets.

4. Hard drive

External clients may have data shipped on a hard drive purchased by the UMGC and invoiced to the client at a cost of $250 per hard drive.

Data Recovery


The UMGC archives most customer data for a year and some datasets are retained for 5 years or more. If you need a dataset re-delivered email a request to next-gen@umn.edu to initiate data recovery. The UMGC does not provide any guarantee that data can be successfully recovered from the archive.

FAQ

Do you accept client-made libraries?


Yes, please consult with UMGC staff before submitting client-made libraries by emailing next-gen@umn.edu.

We recommend that pooling be done at UMGC as PicoGreen concentrations are used for balancing libraries. However, clients may choose to pool themselves.

Client-made libraries will have the following services performed on them before proceeding with sequencing.

  1. PicoGreen (concentration of library)
  2. DNA Agilent High Sensitivity chip (size of library)
  3. KAPA QC (functionality of library)

Where should samples be shipped?


University of Minnesota Genomics Center
1475 Gortner Ave.
28 Snyder Hall
St. Paul, MN 55108
612-625-7736