Sanger Sequencing
The UMGC provides Sanger sequencing on an Applied Biosystems 3730xl DNA Analyzer. This 96-capillary instrument affords rapid, high-throughput service with read lengths of up to 800-1000 bp with performance depending on the quality of the template and the sequence context. For factors affecting Sanger sequencing robustness and data quality, please see our whitepaper.
Our Sanger services are geared to the need for low-cost, rapid, routine sequencing of template-primer reactions assembled by clients (“Classic” service), although we also are able to work with clients to optimize difficult-to-sequence regions.
All services use ABI BigDye Terminator version 3.1 chemistry.
Questions
Contact Anthony Mazza: umgcseq@umn.edu
| Service Option | Repeat reaction if failure |
Include hard copy? |
Includes quantification |
|---|---|---|---|
| Classic Sequencing. Tube Submission. |
Yes | Yes | No |
| Classic. Plate Submission. |
No | No | No |
| Sanger Quality Check and Sequencing. Tube Submission. | Yes | Yes | Yes |
| Sanger Quality Check and Sequencing. Plate Submission. | Yes | No | Yes |
| Clean and Load Only | No | No | No |
| Custom Option Sequencing. | Yes, except for BAC & gDNA |
Yes | No |